摘要
为了探索副鸡禽杆菌的新型分型方法,以肠杆菌基因间共有重复序列(ERIC)和随机核苷酸多态性片段(RAPD)为靶序列进行PCR扩增,对所得指纹图谱用POPGENE和MEGA4.0软件进行遗传距离的计算和聚类分析。结果显示,2种方法均能得到丰富﹑稳定的指纹图谱,其多态性为85.7%(18/21);10个国际标准株、血清C型疫苗株以及4个国内分离株可分为11个谱型,聚为4类。划分的11个谱型可以和现有分型方法中的不同亚型一一对应,提示这种方法可以区分不同血清亚型。表明指纹图谱技术可以有效地区分不同菌株,从而为分子流行病学调查和疫苗研制提供了参考。
In order to explore a subtyping method for Avibacterium paragallinarum,the enterobacte-rial repetitive intergenic consensus（ERICs） and random amplified polymorphic DNAs（RAPDs） were used as the target sequences of PCR to generate a fingerprint of A.paragallinarum. The genetic distance calculation and cluster analysis of the fingerprints were performed by the POPGENE and MEGA4.0 software.The results showed that both methods can get rich,stable fingerprint,with a polymorphism of 85.7%（18/21）. Ten international standard strains,vaccine strains and 5 domestic strains were divided into 11 types and gathered for 4 classes,which corresponded perfectly with subtypes classified with existing serotyping method. The result showed that the fingerprint technology in the present study can distinguish different strains effectively,and therefore provide related information for molecular epidemiological investigation and vaccine development.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第11期1140-1143,共4页
Chinese Veterinary Science
基金
国家"十二五"高技术研究发展计划(863)项目(2011AA10A210)
北京市自然科学基金资助项目(6102009)
北京市优秀人才培养资助项目(2010D002020000009)
