摘要
四川省某藏鸡场藏鸡发生以鼻腔与窦发炎、颜面肿胀、流鼻涕和打喷嚏为主要特征的疾病,通过对送检病鸡病原的分离纯化、生化鉴定、16 S rDNA基因的克隆和序列分析诊断为副鸡嗜血杆菌感染,将该株副鸡嗜血杆菌的16 S rDNA序列与GenBank中15株巴氏杆菌科菌株进行同源性分析发现,与6株副鸡嗜血杆菌同源性较高,为94.2%～97.2%,与其它菌株16 S rDNA基因的同源性较低。另外,药敏试验表明,本菌株对菌必治、阿莫西林、庆大霉素、头孢氨噻肟和阿奇霉素等药物敏感。
An infectious disease occourred in a Tibetan chicken farm in Sichuan province,characterized by sinus inflammation,facial swelling,runny nose and sneezing. Then a bacterial strain was isolated,purified and identified as Haemophilus paragallinarum by biochemical test and sequence analysis of 16 S rDNA gene. Homology analysis revealed that identity of 16 S rDNA sequence between the isolated strain and other six strains of Haemophilus parallinaum was 94.2% to 97.2%. The result of antibiotic sensitive test indicated that this isolated strain was sensitive to ceftriaxone, amoxicillin,gentamicin,cefotaxime and azithromycin.
出处
《中国家禽》
北大核心
2010年第11期22-25,共4页
China Poultry
基金
教育部<长江学者和创新团队发展计划>创新团队项目(IRTO848)
关键词
藏鸡
副鸡嗜血杆菌
分离
16
S
rDNA分析
Tibetan chicken
Haemophilus parallinarum
isolation
16 S rDNA analysis
