摘要
目的:对半固体保存的模拟肛拭子样品进行食源性致病菌的检测,确定引起此次食物中毒的原因,通过实验室间的比对试验,以提高突发公共卫生事件的食源性致病菌的检测能力。方法:根据《食品卫生微生物学检验》GB/T4789.4、7、8、10、30、36-2008、《食品卫生微生物学检验》GB/T4789.5、6、11、14-2003对样品进行增菌分离,观察各平板上的菌落特征,挑取可疑菌落进行生化试验,血清凝集,染色镜检。结果:综合以上得出,本半固体培养基共检出二种菌种:弗氏柠檬酸杆菌;小肠结肠炎耶尔森氏菌。结论:本次食物中毒事件,患者是由于食用了被致病菌污染的食物所引起。
Objective：To detect the simulation of semi-solid rectal swab samples for foodborne pathogens,identify identify the reasons causing the food poisoning,and improve detectability of food-borne pathogens in public health emergencies through inter-laboratory comparison tests.Methods： According to ＂Microbiology Examination for Food Hygiene＂ GB/T4789.4,7,8,10,30,36-2008 and GB/T4789.5,6,11,14-2003,samples were isolated for enrichment,and the colony characteristics were observed on each plate.Suspected colonies were selected for biochemical test,serum agglutination and staining.Results： The above showed that two kinds of bacteria were found in the semi-solid culture base： Citrobacter freundii;Yersinia enterocolitis.Conclusion： In this food poisoning incident,the patients were due to consumption of contaminated food caused by bacteria.
出处
《中国卫生检验杂志》
CAS
2011年第11期2688-2689,2692,共3页
Chinese Journal of Health Laboratory Technology
关键词
肛拭子样品
食源性致病菌
检测
Anal swab samples
Food-borne pathogens
Detection
