摘要
目的:利用水产品中分离到的耐药大肠埃希菌,研究整合子与耐药性的关系。方法:PCR扩增1类和2类整合酶及整合子,将其进行序列分析。结果:85株耐药大肠埃希菌中88%含有1类整合酶基因;其中68%能扩增此基因;1类整合子可变区PCR扩增产物谱型有17种;1类整合子最常见的基因盒有4种。还发现1株大肠埃希菌同时携带1和2两类不同的整合酶和整合子。结论:大肠埃希菌耐药性与整合子有相关性。
Objective：Escherichia coli strains with antibiotic resistance isolated from some fishery products were used to investigate the relationship between the integrons and the antibiotic resistance profile.Methods： PCR method was performed to amplify class 1 and class 2 integrases（intI1 and intI2） as well as integrons for sequencing analysis.Results： It was found that 88% of 85 E.coli strains with antibiotic resistance contained class 1 integrase gene,in which,68% can amplify this gene.There were 17 different PCR amplicon profiles in variable region of class 1 integrons.Four kinds of gene cassettes with antibiotic resistance were frequently detected.Besides,1 E.coli strain carried both class 1 and class 2 integrases and integrons.Conclusion： There was inter-relationship between antibiotic resistance and integrons in Escherichia coli strains.
出处
《中国卫生检验杂志》
CAS
2011年第11期2609-2611,2615,共4页
Chinese Journal of Health Laboratory Technology
基金
苏州市"科教兴卫"青年科技项目(SWKQ0825)
关键词
水产品
大肠埃希菌
整合子
基因盒
Fishery products
Escherichia coli
Integron
Gene cassette
