摘要
采用薄膜-超声法制备丁香酚脂质体,利用正交实验优化出最佳处方和最优制备工艺,以包封率和粒径形态及大小为质量评价目标,以萃取法结合紫外分光光度法测定丁香酚脂质体包封率,透射电镜观察其粒径形态及大小。试验结果表明丁香酚脂质体最佳制备工艺和处方为:胆脂比1:6;药脂比1:5;超声时间40min;制备的脂质体粒径均匀,呈球形小囊泡,包封率为(88.3±3)%。
The liposome of eugenol was prepared with the film dispersion-supersonic method,and,the orthogonal design was used to optimize the prescription and preparation technology of eugenol liposome.The result of preparation was evaluated with the encapsulation rate which it was determined by UV spectrophotometry after extraction from eugenol liposome.The appearances and the particle diameter of eugenol liposome were examined with transmission electron microscopy.The optimum prescription and preparation of eugenol liposome were 1：6 （proportion of Cholesterin to Phosphatidylcholine）,1：5 （proportion of eugenol to Phosphatidylcholine）,40 min （ultrasonic time）.The encapsulation rate of eugenol liposome was （88.3±3）%.The particle diameter of eugenol liposome was about 200 nm and its appearance was uniformity globular vesicle.
出处
《中国农学通报》
CSCD
北大核心
2011年第26期79-82,共4页
Chinese Agricultural Science Bulletin
基金
贵州大学研究生创新基金"丁香酚脂质体的制备及对鱼类麻醉作用和体外抗菌作用的研究"(2010019)
关键词
丁香酚
脂质体
制备
eugenol
liposome
preparation
