摘要
欧李[Cerasus humilis(Bge.) Sok.]为蔷薇科樱桃属果树,别名钙果,原产中国,分布于我国的黑龙江、辽宁、内蒙古、河北、山东、山西等省区。多生长在向阳山坡或沙丘边缘,资源相当丰富。欧李株高0.3～1.5 m,多为0.5～0.7 m左右,是目前世界上最矮小的木本果树。果实可鲜食或加工,含糖、蛋白质、维生素C,特别是矿质元素铁和钙的含量很高,每100果肉干分别含有58和360 mg种仁可作药用,中药称之为"郁李仁"可消毒化肿。欧李成花容易,根蘖更新能力强,抗逆性强[1]。
One serious disease happened on the fruit of Chinese Dwarf Cherry in Liaoning Province recently.The typical symptom was brown spot formed on the fruit surface.The spot spread quickly through the whole fruits,then the fruits were rotten.There were tomentum round shaped mildew formed on the surface of sym-ptomatic fruits.The pathogen was isolated from infected fruits.After pathogenicity tests in lab and field and re-isolation,the isolate H1 was determined to be responsible for the disease.H1 colony on PDA was 70-75 mm in diameter,with concentric rings in grayish or light brown color after 7 days incubation at 22℃ with illumination of 12 h near-UV/12 h dark.The conidia were one-celled,hyaline,lemon-shaped,（10-27） μm×（7-17） μm on PDA,produced in branched monilioid chains.The rDNA ITS sequence of islolates H1 and G1 had 100% similarity with those of Monilinia fructicola in GenBank.The amplification results with three Monilinia specific primer pairs showed that only primer pair ITS1Mfcl and ITS4Mfcl could amplify a 356 bp fragment.Based on the morphological characteristics and rDNA molecular analysis,the pathogen was finally identified as M.fructicola.
出处
《植物病理学报》
CAS
CSCD
北大核心
2011年第6期626-630,共5页
Acta Phytopathologica Sinica
关键词
欧李
褐腐病
病原菌
鉴定
Chinese Dwarf Cherry
Monilinia fructicola
morphological identification
ITS sequence analysis
specific amplification
