摘要
本试验通过NBF液固定,阿尔新蓝—藏红O复染(AB/SO)法与吖啶橙染色(Acridine-Orange,AO),对不同日龄鸡免疫器官中肥大细胞(MC)及肝素阳性细胞的形态、分布及数量变化进行观察分析。结果显示,NBF溶液固定,AO染色可以清晰地显示肝素阳性细胞,其呈圆形、椭圆形和不规则形,大小不一;胸腺中肝素阳性细胞主要分布在髓质;法氏囊中肝素阳性细胞多分布于淋巴小结周围;脾脏中肝素阳性细胞分布在实质,血窦、血管周围偶见;肝素阳性细胞与AB/SO染色显示的MC的分布十分相似;18日胚龄前,免疫器官中肝素阳性细胞的数量与胚龄呈正相关,18日胚龄后肝素阳性细胞数量下降是否与肥大细胞脱颗粒有关,尚不清楚。
For observing and analyzing the morpha, distribution and quantity of mast cells （MC）and heparin positive masccline cells,the differentiation time and degree of immune organs in different age of chick embryos were fixed by neutro-formalin, stained by AB/SO and Acridine-Orange（AO）. The result showed that the samples fixed in neutro-formalin and stained by AO could clearly displayed organizational structure of heparin positive masccline cells,which were circular, oval and anomalo under light microscopy. Numerous heparin positive masccline cells were concentrated in the thymus medulla. Heparin positive masccline cells in bursa of Fabricius were occasionally seen at the edge of lymphatic nodules. Heparin positive masccline cells of spleen were uniformly distributed in parenchyma, in blood sinuses and blood vessels sometimes. The distribution of heparin positive masccline cells and MC stained by AB/SO were exactly similar. The relation between the number of heparin positive masccline cells and embryo age was positive correlation before the 18th day. After that,whether decline in the number of heparin positive masccline cells was correlated with degranulation was unclear.
出处
《中国家禽》
北大核心
2011年第22期24-27,共4页
China Poultry
基金
贵州省科学技术基金资助项目[黔科合J字(2009)2132]
关键词
鸡胚
肝素阳性细胞
肥大细胞
免疫器官
chicken embryo
heparin positive masccline cells
mast cells
immune organs
