摘要
目的探讨不同浓度氯胺酮对成人辅助性T淋巴细胞（Th细胞）分化的影响。方法择期手术患者20例，年龄20～60岁，ASA分级Ⅰ级，麻醉前从每位受试者肘静脉抽取血样20ml，分离外周血单个核细胞（PBMCs）。采用随机区组设计，将每份血样分离出的PBMCs随机分为3组（n=20）：氯胺酮不同终浓度组（K，组和K2组终浓度分别为2．5、25．0μg／ml），正常对照组（C组）加入等容量0．9％NaCl。24h后分别加入植物血凝素刺激，继续孵育48h后收集细胞。采用流式细胞小球微阵列术检测细胞培养上清液中IL-2、IL4、IL-6、IL-10、干扰素-γ（IFN-γ）和TNF-α的浓度；采用四色荧光流式细胞技术（CD3^＋CD8^-IFN-γ^＋标识Th1细胞，CD3^＋CD8^-IL-4^＋标识Th2细胞）测定Th1及Th2细胞亚群比例，并计算二者比值（Th1／Th2）。结果C组、K1组和K2组Th1及Th2细胞亚群比例、Th1／Th2、细胞培养上清液中IL-2、IL4、IL-6、IL-10、IFN-γ和TNF-α的浓度差异无统计学意义（P〉0．05）。结论镇静浓度和麻醉浓度的氯胺酮在体外环境下对成人Th细胞分化无影响。
Objective To investigate the effects of different concentrations of ketamine on the differentiation of human T helper （Th） cells. Methods Twenty patients ASA Ⅰ , 20-60 years undergoing elective operation under general anesthesia were enrolled in this study. Peripheral venous blood samples were taken before anesthesia. Peripheral blood mononuclear cells were isolated and assigned to one of 3 groups （ n = 20 each）. Peripheral blood mononuclear cells were incubated in the presence of 0.9 % NaCL （group C）, 2 concentrations of ketamine （2.5, 25.0 μg/ml） （groups K1, K2 ） for 24 h, and were then stimulated with phytohaemagglutinin for another 48 h. The percentage of Thl and Th2 ceils were detected by four-color flow cytometry. The Th1/Th2 ratio was calculated. The IL-2, IL-4, IL-6, IL-10, IFN-7 and TNF-α concentrations in the supernatant were determined by cyto- metric bead array. Results There was no significant difference in the IL-2, IL-4, IL-6, IL-10, IFN-γ and TNF- α concentrations in the supernatant, the percentage of Thl and Th2 cells and Thl/Th2 ratio among groups C, K1 and K2 （ P 〉 0.05）. Conclusions The sedative and anesthetic concentrations of ketamine exert no effect on the differentiation of human Th cells in vitro.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第8期916-918,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（30801068）
关键词
氯胺酮
T淋巴细胞
细胞分化
Ketamine
T-lymphocytes
Cell differentiation
