摘要
Objective To observe mechanisms of electroacupuncture （EA） on treating Parkinson＇ s disease （PD）. Methods Fifty SD rats were randomly divided into 4 groups： a normal control group （Norm）, a sham operation group （Sham）, a model group （Mod） and an electroacupuncture （EA） group. EA at ＂Fengfu＂ （风府 GV16） and ＂Taichong＂ （太冲 LR 3） was applied once daily for 2 weeks in Group EA, and no treatment was given to the animals in the other three groups. PD model was established by using 6-hydroxydopamine. Numbers of tyrosine hydroxylase （TH）, tumor necrosis factor-alpha （TNF-α）and interleukin-1 beta （IL-1β） positive reactive cells in substantia nigra （SN） were counted by using immunohistochemical methods. Results The total TH positive neurons in Group Mod were obviously less than those in Group Norm and Group Sham （P〈0.01）, and the numbers of TNF-α and IL-1β positive cells in Group Mod increased more significantly than those in Group Norm, and Group Sham （P〈0.01）. The total TH positive neurons in Group EA were obviously more than those in Group Mod （P〈0.01）, and the numbers of TNF-α and IL-1β positive cells in Group EA were obviously less than those in Group Mod （P〈0.01）, indicating that EA could markedly lower the contents of such pre-inflammatory factors like TNF-α and IL-1β in SN of experimental rats with PD. Conclusion EA may provide its protective functions on dopaminergic neurons by way of alleviating inflammatory reactions in SN of rats with PD.
目的:探讨电针治疗帕金森病(PD)的作用机制。方法:取SD大鼠50只,随机分为正常组、假手术组、模型组和电针组。电针组针刺"风府""太冲",每日1次,共治疗2周,其它组不予治疗干预。以6-羟基多巴胺制备PD大鼠模型。采用免疫组织化学法观察黑质酪氨酸羟化酶(TH)、肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)阳性细胞数量。结果:模型组损毁侧黑质TH阳性神经元数量显著低于正常组和假手术组(P<0.01),TNF-α和IL-1β阳性细胞数量显著高于正常组和假手术组( P<0.01);电针组黑质TH阳性神经元数量显著高于模型组(P<0.01),TNF-α、IL-1β阳性细胞数量显著低于模型组(P<0.01)。结果表明,电针治疗能显著降低PD模型大鼠黑质TNF-α、IL-1β等前炎性反应因子含量。结论:电针干预可能通过减轻PD模型大鼠黑质内的炎性反应,而对多巴胺能神经元施加保护作用。
基金
Supported by National Projects of Natural Science Foundation: 30300461, 30973787, 30973809
Project of Science and Technology of Education Department of Hubei Province: 2002 B 00004, D 200726001, Z 20091601
Project of Science and Technology of Hubei Science and Technology Agency: 2009 CDA 068, 2010 CDA 101
Project of Science and Technology of Wuhan Science and Technology Agency: 201060623269
