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维吾尔族宫颈鳞癌C/EBP β基因甲基化与HPV16感染关系 被引量：5

Methylation of C/EBP β gene and infection of HPV16 in cervical squamous cell carcinoma among Uigur women
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摘要 目的通过对新疆维吾尔族妇女宫颈鳞癌组织C/EBPβ基因甲基化与HPV16感染关系的研究,探讨该基因在宫颈癌发生发展过程中的作用。方法提取26份子宫颈癌组织和16份子宫颈正常组织DNA,用PCR方法对样本进行HPV16检测,再利用基质辅助激光解析电离飞行时间质谱方法进行C/EBPβ基因甲基化分析。结果子宫颈鳞癌组织HPV16感染率为69.32%(18/26),2份正常子宫颈组织感染HPV16,差异有统计学意义(χ2=12.780,P<0.05);子宫颈鳞癌与子宫颈正常组织中C/EBPβ基因CpG10/11和CpG17/18 2个CpG岛甲基化差异有统计学意义(t=2.221、2.278,P均<0.05);C/EBPβ基因在以上2个CpG岛位点上的甲基化率与HPV16感染偏相关系数分别为1.323和-4.36,差异无统计学意义(P>0.05)。结论新疆维吾尔族妇女宫颈鳞癌发生与HPV16感染相关;C/EBPβ基因2个CpG岛位点甲基化与维吾尔族子宫颈鳞癌发生相关,而与HPV16感染无相关性。 Objective To investigate the function of C/EBP β gene in the development of cervical squamous cell carcinoma by analyzing methylation of C/EBP β gene and infection of human papillomavirus type 16（HPV16） in cervical squamous cell carcinoma among Uigur women. Methods DNA was extracted from 26 cervical squamous cell carcinoma tissue and 16 normal cervical tissue samples.HPV16 was detected in cervical squamous cell carcinoma tissue and normal cervical tissue by PCR method.The C/EBP β gene methylation was analyzed with matrix assisted laser desorption ionization-time of flight（MALDI-TOF） mass array method. Results The HPV16 infection rate of cervical squamous cell carcinoma was 69.32﹪（18/26） and HPV16 infection rate of normal cervical tissues was 12.5%（2/16） with a significant difference between the two groups（χ2=12.780,P0.05）.The sites of CpG10/11 and CpG17/18 islands on C/EBP β gene methylation in cervical carcinoma were significant higher than those of the normal cervical tissue according to two-sample t-test（t=2.221,P0.05;t=2.278,P0.05）.The correlation coefficients beween the sites of CpG10/11 and CpG17/18 CpG islands on C/EBP β gene methylation and HPV16 infection were 1.323 and-4.361,respectively,without significant difference（P0.05）. Conclusion HPV16 infection is associated with cervical carcinoma incidence among Uigur women.The sites of CpG10/11 and CpG17/18 on C/EBP β gene are related with cervical squamous cell carcinoma in Uigur women and methylation rate is not correlated with HPV16 infection.
作者 蒋旭鹏 潘晓琳 潘泽民 张东辉 张鑫芝
机构地区 石河子大学医学院生物化学教研室 石河子大学医学院新疆地方与民族高发病教育部重点实验室
出处 《中国公共卫生》 CAS CSCD 北大核心 2011年第11期1409-1410,共2页 Chinese Journal of Public Health
基金 国家自然科学基金(30860302 30660193) 教育部春晖计划资助(Z2006-1-83011)
关键词 C/EBPβ基因 子宫颈鳞癌 HPV16感染 基因甲基化 维吾尔族妇女 C/EBP β gene cervical squamous cell carcinoma HPV16 infection gene methylation Uigur women
分类号 R737.33 [医药卫生—肿瘤]
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共引文献280

同被引文献36

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中国公共卫生
2011年 第11期

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