摘要
作者应用限制性内切酶ＨｐａⅡ和ＭｓｐⅠ酶切胃癌组织及正常胃组织ＤＮＡ，经ＰＣＲ扩增ｐ５３基因第５外显子，琼脂糖凝胶电泳分析其电泳图谱，比较胃癌组织及正常胃组织ｐ５３基因第５外显子特定序列５′－ＣＣＧＧ－３′位点甲基化差异。结果显示：１５例胃癌组织中１２例ｐ５３基因第５外显子出现高甲基化状态，而１０例正常胃组织为低甲基化状态，结果提示，ｐ５３基因高甲基化状态与胃癌发生有关。
The methylation of the Exon 5 of p53 gene was analyzed by PCR using restriction endonuclease enzymes Hpa Ⅱ and Msp Ⅰin 15 gastric cancer Tissues and 10 normal gastric tissues. The result showed that 5′CCGG3′ site was methylated at the exon 5 of p53 gene in 12 gastric cancer tissues, While hypomethylation was exist in 10 normal gastric tissues. It is suggested that hypermethylation of p53 gene plays a important role in gastric carcinogenesis and mutation of p53 gene.
出处
《实用癌症杂志》
1998年第1期19-20,共2页
The Practical Journal of Cancer
