摘要
目的:体外观察三氧化二砷(As2O3)诱导人类腺样囊性癌高转移株(ACC-M)细胞凋亡过程中的作用,同时检测此过程中ACC-M内凋亡抑制基因Survivin表达水平的变化。方法:MTT法检测不同浓度As2O3作用不同时间对ACC-M细胞的抑制效应;流式细胞术观察As2O3诱导各组ACC-M细胞的凋亡率。RT-PCR检测Survivin基因mRNA的表达变化;Western blot检测判定Survivin蛋白水平的表达差异。结果:As2O3可明显抑制ACC-M细胞的生长,其抑制率呈浓度和时间依赖关系,细胞凋亡率也呈相同的趋势。RT-PCR和Western blot检测显示As2O3作用下,ACC-M内Survivin mRNA和蛋白表达明显受抑制,抑制程度也具有时间和剂量依赖性。结论:As2O3可通过促进细胞凋亡的方式使体外培养的ACC-M细胞生长受抑制,而受抑制的ACC-M内Survivin mRNA和蛋白表达均下降,推测Survivin基因在As2O3诱导的ACC-M细胞凋过程中起着重要作用。
Objective： To investigate the proliferation inhibition of arsenic trioxide（As2O3） on salivary adenoid cystic carcinma-M（ACC-M） cells in vitro and to study the role of Survivin on the apoptosis of ACC-M induced by As2O3.Methods： ACC-M cells were treated with different concentration of As2O3 for different periods.The inhibitory effects on cell＇s growth were assayed with MTT test.Apoptosis was determined by flow cytometry.The expression of Survivin mRNA and protein were investigated by RT-PCR and Western blot analysis respectively.Results： Cell viability after As2O3 treatment was markedly suppressed and exhibited as a dose-and time-dependent pattern.The apoptotic index showed the similar trend.The results of RT-PCR revealed gene expression of Survivin was suppressed significantly.Through Western blot analysis,a negative correlation between concentration and amount of protein product of Survivin was determined.Conclusion： As2O3 might markedly suppressed ACC-M cell＇s viability in vitro.The inhibition of Survivin gene expression may play a critical role on ACC-M cell apoptosis induced by As2O3.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2011年第10期911-914,共4页
Journal of Oral Science Research
