摘要
获得了粳型水稻77-170品系原生质体再生植株(R_2)6个株系的206棵后代植株。对其中96株进行性状观察和细胞学染色体鉴定,发现再生植株子代在株高、剑叶和主穗长、单株有效穗数、每穗粒数、育性、生育期等性状上都产生了变异,除株高外其他性状在第2代遗传上不稳定,染色体倍性稳定(2_n=24)。对56株再生植株子代作酯酶、过氧化物酶同工酶测定,其酶谱谱带与对照实生株相似。
A total number of 206 regenerated plants (R2) of 6 plant lines of rice strain 77-170 from protoplasts were obtained. 96 plants out of them were observed their the genetic character and chromosome number. The variation of characters was shown in the height of plant, length of flag leaf and main panicle, number of effective panicle and grains per panicle, fertility and growth duration of the regenerated plants (R2). Except the plant height, all of characters were unstable in R? The chromosome number was 24 (2n = 24). 56 plants out of them were tested with isozyme. The isozyme zymograms of esterase and peroxidase of seeds of the regenerated plants (R2) were similar to those of the control plants.
关键词
水稻
原生质体
再生植株
后代
性状
Rice, Protoplast, Regenerated plant, Progeny, Character, Esterase, Pero-xidase
