摘要
研究了不同培养方法、激素种类及质量浓度、抗生素种类及质量浓度对转基因烟草K326外植体分化的影响,并分别对其进行比较。结果表明:在培育转基因烟草K326抗性外植体的不同时期,依次选用最佳配方的烟草叶盘共培养培养基MS+1 mg/L 6-BA+0.1 mg/L NAA、烟草滤菌培养基MS+1 mg/L 6-BA+0.1 mg/L NAA+500 mg/L Carb、烟草筛选培养基MS+100 mg/LKan、烟草继代培养基MS+80 mg/L Kan+300 mg/L Carb、烟草生根培养基MS+0.1 mg/L NAA+300 mg/L Carb对转基因烟草外植体进行培育,可使转基因烟草外植体分化迅速,获得质量优良的试验材料,为进一步培育得到理想的转基因烟草K326完整植株奠定了工作基础。不同培养条件对外植体分化有较显著的影响,28℃、16 h光照最利于转基因烟草K326的外植体分化生长。
This research was to study the influence of different cultivation methods,hormones and antibiotics on explant differentiation of transgenic tobacco.There were five different culture media for the research,including MS＋1 mg/L 6-BA＋0.1 mg/L NAA for co-cultivation,MS＋1 mg/L6-BA＋0.1 mg/L NAA＋500 mg/L Carb for sterilization cultivation,MS＋100 mg/L Kan for screening,MS＋80 mg/L Kan＋300 mg/L Carb for subculture,and MS ＋0.1 mg/L NAA＋300 mg/L Carb for root induction.With these mediums the explant of transgenic tobacco K326 could differentiate rapidly,which laid the basis for further cultivation of the whole plants of transgenic tobacco K326.
出处
《河南农业科学》
CSCD
北大核心
2011年第10期52-55,共4页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(30771301)
教育部留学回国人员科研启动基金项目
关键词
转基因烟草
外植体
培养基
转化
共培养
Transgenic tobacco
Explant
Culture medium
Transformation
Co-cultivation
