摘要
以野生型拟南芥花蕾中提取的总RNA为模板,通过RT-PCR的方法扩增到与角质和蜡质合成相关的转录因子基因,该目的基因片段SHN1/WIN1(SHN1/WAX INDUCER1)约600 bp,将此片段克隆到PMD-19T载体上,经测序分析与Genbank中报道的序列的同源性为100%。以植物表达载体PBI121为基础,构建了由组成型启动子CaMV35S调控的SHN1/WIN1基因的植物表达载体PBI121-SHN1/WIN1,为利用SHN1/WIN1基因改变植物角质膜的结构和成分,提高植物抗逆性特别是抗旱性能奠定了物质基础。
The SHN1/WIN1 gene with about 600 bp amplified by reserve transcription polymerse chain reaction （RT-PCR） from wide Arabidopsis thaliana flower buds was cloned on PMD-19T vector. The sequence analysis showed that the homology of the SHN1/WIN1 gene identified with SHN1/WIN1 in Genbank. The plant expression vector PBI121-SHN1/WIN1 of SHN1/WIN1 gene was constructed, which laid a foundation for improving plant adverse resistance especially drought resistance by using SHN1/ WIN1 gene in order to change the construction and composition of plant cuticle.
出处
《贵州农业科学》
CAS
北大核心
2010年第4期26-29,共4页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目"作物表面角质膜渗透吸收性能的研究"(30771301)
教育部留学回国人员科研启动基金项目"高效农药助剂(渗透剂)的研究和筛选"
