摘要
目的观察不同强度的恒磁场作用不同时间对培养的人脐静脉内皮细胞（HUVECs）增殖、凋亡及分泌功能的影响。方法将HUVECs分别暴露于场强为5、22、86或135mT的恒磁场中，磁场作用时间分别为8、12或24h。以流式细胞技术分析恒磁场对HUVECs增殖及凋亡的影响；比色法测定细胞培养液中一氧化氮（NO）含量的变化；放射免疫法测定细胞培养液中的内皮素-1（ET-1）及6-酮-前列环素F1α（6-keto-PGF1α）的含量。结果①低强度磁场（5mT）短时间（8h）作用于HUVECs可促使其增殖，但高强度磁场（135mT）或作用时间过长（12h或24h）则会抑制HUVECs的增殖；②磁场对HUVECs凋亡无影响；⑧低强度磁场（5mT）短时间（8h）作用可促使HUVECs合成释放NO和6-keto—PGF1α但高强度磁场（135mT）或作用时间过长（12h或24h）则会抑制HUVECs合成分泌NO和6-keto—PGF1α；④细胞暴露于磁场8h时，5mT组和22mT组与对照组比较，HUVECs合成释放ET-1量无明显变化（P〉0．05），但86mT组和135mT组的细胞ET-1分泌量高于对照组（P〈0．01）。当磁场作用时间分别为12和24h时，5mT组与对应时间点对照组比较，细胞ET-1合成释放量仍无变化（P〉0．05），22mT组、86mT组、135mT组则明显低于对照组（P〈0．01）。结论低强度磁场、短时间作用可促进HUVECs的增殖及舒血管物质的合成分泌，而高强度磁场或磁场作用时间过长则抑制HUVECs增殖，增加缩血管物质的合成。
Objective To evaluate the effects of static magnetic fields （SMFs） of different intensity and exposure duration on the proliferation and apoptosis of human umbilical cord endothelial cells （HUVECs） , and their release of nitric oxide （NO） , 6-keto-prostaeyclin lct （6-keto-PGF1α） and endothelin （ ET-1 ）. Methods Cultured HUVECs were exposed to a SMF at5, 22, 86 or 135 mT for8, 12 or 24 hours. Their proliferation and apoptosis were monitored by flow cytometry （ FCM ）. The medium was collected to test its NO content by optical density. ET-1 and 6-keto-PGF1α were measured by radioimmunization. Results （ 1 ） The proliferation of HUVECs increased when the cells were exposed to a SMF at 5 mT for 8 h, but a SMF at 135 mT for 12 h or 24 h inhibited the proliferation of HUVECs. （2） An SMF had no effect on apoptosis of HUVECs. （3） An SMF at 5 mT for 8 h increased the release of NO and 6-keto-PGFla, but the release of NO and 6-keto-PGF1α decreased when the SMF intensity was 135 mT or the cells were exposed to an SMF for 12 h or 24 h. （4） An SMF at 5 mT or 22 mT for 8 h did not effect the release of ET-1. An SMF at 86 mT or 135 mT increased the release of ET-1. Compared with a control group, an SMF at 5 mT for 12 or 24 h did not affect the release of ET- 1 , but at 22, 80 or 135 mT, the release of ET-1 decreased significantly. Conclusions Exposure to a low intensity SMF for a short duration could improve the proliferation of HUVECs and increase the release of vasoaetive factors, but if HUVECs are exposed to a strong SMF or exposed for a long duration, the proliferation and the re- lease of vasoaetive factors is decreased.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第10期742-745,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金资助项目（30900290）
关键词
恒磁场
内皮细胞
一氧化氮
内皮素-1
6-酮-前列环素
Static magnetic fields
Endothelial cells
Nitric oxide
Endothelin-1
6-keto-prostacyclin 1α
