摘要
目的研究广西瑶族人群血小板抗原HPA-1～17系统基因多态性分布特点。方法采用PCR-序列特异性引物(PCR-SSP)方法对48例广西瑶族健康人进行HPA-1～17系统基因分型,计算基因型频率、等位基因频率,并与其他种族、地区人群相关资料比较。结果在17个HPA系统中,HPA-3基因型杂合频率最高,HPA 3a/3a、HPA3a/3b和HPA 3b/3b的频率分别为0.2292,0.5208和0.2500;HPA-15的杂合程度仅次之,HPA 15a/15a,HPA 15a/15b和HPA15b/15b的频率分别为0.3125、0.4583和0.2292;其余HPA系统只检测到a等位基因,其基因频率均为1.000,符合Hardy-Weinberg遗传定律。结论 HPA基因多态性分布存在明显的种族和地域性差异。广西瑶族HPA-3和HPA-15系统基因频率分布具有多态性,在临床血小板输注中必须重视。
Objective To analyze the regional distribution feature of gene polymorphism of human platelet antigen （HPA） 1 - 17 system in Yao ethnic group in Guangxi. Methods HPA 1 - 17 system genotyping of 48 Yao ethnic group was conducted by using PCR-SSP and the genotype frequency and gene frequency were calculated. Results Among the 17 HPA systems, HPA-3 had the greatest heterozygosity with a gene frequency of 0. 2292,0.5208 and 0. 2500 for HPA-3a/3a, HPA-3a/3b, HPA-3b/3b, respectively;followed by HPA-15,which showed the heterozygosity with a gene frequency of O. 3125,0. 4583 and 0. 2292, respectively. The predominance of a/a homozygosity was noted for HPAI-17, and the frequency of a/a homozygosity was 1.000 for the remaining 17 I-IPAs. There was a good fit to Hardy-Weinberg equilibrium within each group. Conclusion The ethnic and original difference is significant in HPA. HPA-3 and HPA-15 have their own characteristic in Guangxi Yao population. The distribution of HPA-3 and HPA 15 gene frequencies are polymorphous, their roles in clinical platelet matched transfusion should be paid attention to.
出处
《广西医学》
CAS
2011年第12期1572-1574,共3页
Guangxi Medical Journal
基金
广西南宁市科学研究与技术开发计划项目(201106032C)
关键词
血小板抗原基因
多态性
瑶族
广西
Hmnan plateletantigen
Polymorphism
Yao nationality
Guangxi
