摘要
目的通过黄参多糖(SGP)、游泳运动对采用高脂饮食建立的营养性肥胖小鼠血脂及瘦素水平进行干预,探索SGP及游泳运动的降脂减肥作用。方法本实验应用酶法测定了血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)﹑高密度脂蛋白(HDL-C)和应用酶联免疫法(ELISA)测定了小鼠血清瘦素,称取生殖器周围脂肪质量,计算肥胖指数(Lee's index)和脂肪系数。结果高脂组(HFD)与基础对照组(Cont)比,体质量增加,Lee'指数和脂肪系数差异达到显著,证明通过高脂饮食建立营养性肥胖模型是成功的;应用SGP或游泳运动或二者联用均能降低营养性肥胖小鼠的体质量、体脂,降低血清中TC、TG及LDL-C,升高HDL-C水平,瘦素水平也随之在减肥中发挥作用而逐渐接近正常,其中以SGP和游泳运动联用产生协同作用效果最佳,与HFD组比差异达到极显著(P<0.01)。结论 SGP或游泳运动均能使营养性肥胖小鼠降脂、减肥,尤其是二者联用效果极为明显,为其在实践中应用提供了科学依据。
Objective Through intervenient study of sphallerocarpus gracilis polysacchorides （SGP） and swimming on levels of serum lipid and leptin in nutritional obese mice were established by high-fat diet, the function of lipid reduction and weight loss by SGP and swimming was explored. Method The blood total cholesterol （TC）, triglycerides （TG）, low-density lipopro- tein （LDL-C）, high-density lipoprotein （HDL-C） were tested by enzyme and leptin was measured by ELISA in this experiment. Meanwhile, the mice fat tissues in the genital were removed and weighed, and lee＇ s index and fatty coefficient were counted. Results Compared with normal control group, the results showed the weight of mice which were feed high fat diet was increased, and lee＇ s index and fatty coefficient were significantly increased. This proved that nutritional obese model which was feed high fat diet was succeeded. And the weight, body fat, TC, TG and LDL-C of nutritional obese mice were decreased by SGP, swimming or SGP-swimming combination was introduced. The level of HDL-C was rose and the level of leptin was gradually decreased and similar to the normal level, especially the effect by SGP-swimming combination had the best which were decreased significantly compared with HFD group （ P 〈 0.01 ）. Conclusion Nutritional obese mice had reduced weight and serum lipid by SGP or swimming, especially the effect by SGP-swimming combination had the best. Therefore, this research could provide scientific reference for application in practice.
出处
《西安体育学院学报》
CSSCI
北大核心
2011年第6期710-714,共5页
Journal of Xi'an Physical Education University
基金
安徽省教育厅自然科学项目(KJ2009B274Z)
黄山学院引进人才启动项目资助(2008xskq013)
关键词
黄参多糖
游泳
血脂
瘦素
减肥
sphallerocarpus gracilis polysacchorides （SGP）
swimming
serum lipid
leptin
weight loss
