摘要
目的探讨神经营养素-3(NT-3)基因修饰雪旺细胞(SCs)和NT-3受体(TrkC)基因修饰神经干细胞(NSCs)联合移植能否减少大鼠脊髓全横断损伤处的瘢痕组织,并促进下行神经纤维再生。方法用NT-3基因的重组腺病毒(Ad-NT-3)感染培养的SCs(NT-3-SCs),同时用含TrkC基因的重组腺病毒(Ad-TrkC)感染培养的NSCs(TrkC-NSCs)。此外,还将Ad-LacZ分别感染SCs和NSCs。然后建立大鼠脊髓全横断损伤模型,将NT-3-SCs+TrkC-NSCs联合移植到损伤处。所有动物存活60 d后,在脊髓横断处远端3 mm组织内注射荧光金(FG),动物再存活7 d。最后灌注固定动物、取材和切片,并行形态学观察。结果①在移植组中,脊髓横断处头侧端、中脑红核及大脑体感运动区皮质内锥体细胞层有少量FG标记神经元。②在脊髓损伤区内,细胞移植组比对照组的纤维粘连蛋白(FN)阳性细胞减少,S-100阳性细胞增多。③电镜下可见,细胞移植组的脊髓损伤区内成纤维细胞和胶原纤维减少。结论 NT-3基因修饰SCs和TrkC基因修饰NSCs联合移植能够减少大鼠脊髓全横断损伤处的瘢痕组织,促进下行神经纤维的再生。
Objective To study whether co-transplantion of NT-3 gene modified SCs and TrkC gene modified NSCs could reduce the formation of scar tissue and promote the regeneration of descending nerve fibers in the lesion of spinal cord.Methods SCs（NT-3-SCs） were infected by recombinant adenovirus vector carrying NT-3 gene（Ad-NT-3）.In the meanwhile,NSCs（TrkC-NSCs） were infected by recombinant adenovirus vector carrying TrkC gene（Ad-TrkC）.Some SCs and NSCs were respectively infected by recombinant adenovirus vector carrying LacZ gene（Ad-LacZ）.Then the model of completely transected spinal cord injury was established in adult Sprague-Dawley rats.NT-3-SCs＋TrkC-NSCs were grafted into the transected site of spinal cord.60 days after the transplantation,fluorogold（FG） was injected into the spinal cord 3 mm caudal to the transected site.7 days after injected FG,animals were then sacrificed and fixed.Section and morphological serial studies were performed.Results ①In the transplant group,there were some FG-labeled neurons in the rostral spinal cord nearby the transected site,the red nuclei and the internal pyramidal layer of sensorimotor cortex.②In the cell tranplant group there are less fibronection positive cells and more S-100 positive cells than control group.③Electron microscope showed the less fibroblast and collagenous fibre bundlec in injured spinal cord in the grafted cells groups.Conclusion Co-transplantation of NT-3 gene modified SCs and TrkC gene modified NSCs may reduce the formation of scar tissue and promote the regeneration of descending nerve fibers in the lesion of spinal cord.
出处
《解剖学研究》
CAS
2011年第5期367-372,共6页
Anatomy Research
基金
国家自然科学基金(30270700)
广东省社会发展攻关基金(2003C33808)
