摘要
目的探究维甲酸对颅咽管瘤存化细胞凋亡诱导作用.方法获得临床颅咽管瘤手术切除新鲜组织,通过原代培养技术获得实验细胞,体外维甲酸处理后应用Annexin V,DAPI染色和DNA ladder检测细胞凋亡.结果维甲酸处理组比对照组(为处理组)明显体现出更多的细胞磷脂外翻Annexin V强染,表现为早期凋亡状态.同时实验组相比对照组DAPI强染,明亮,DNA ladder实验阳性,呈中晚期凋亡状态.结论 20μmol维甲酸处理原代颅咽管瘤细胞能有效诱导其进入早期凋亡,对颅咽管瘤细胞有杀伤作用.
Objective To explore the inducing effects of RA on the apotosis of primary cultured craniopharyngiomic cells.Methods Caniopharyngiomic cells were pimary cultured from fresh surgical samples,and then were treated with 20 mol RA for east 24 hours.Then these cells were collected for apoptotic tests which included Annexin V,DAPI staining and DNA ladder.Results RA treated primary cells presented higher level of Annexin V than untreated cells.Meanwhile DAPI staining indicated RA incubation leaded to pyknosis and nuclear condensation.In consistent,DNA ladder experiments were positive in RA treated primary cultured craniopharyngiomic cells.The results showed RA induced apoptosis of caniopharyngiomic cells.Conclusion 20 μmol of RA can successfully induce apoptosis of primary cultured craniopharyngiomic cells in vitro after 24 hours incubation,so as to kill craniopharyngiomic cells by initiating the apoptosis process.
出处
《昆明医学院学报》
2011年第9期12-15,共4页
Journal of Kunming Medical College
基金
云南省科委专项研究基金资助项目(2008CD038)
