摘要
目的观察血管内皮生长因子(VEGF)-C和趋化因子受体CCR7在卵巢癌组织内的表达情况,分析VEGF-C和CCR7的表达与癌淋巴结转移之间的关系。方法取卵巢癌病例72例,其中,淋巴结转移组46例,无淋巴结转移组26例。应用免疫组化技术观察VEGF-C和CCR7在卵巢癌组织内的表达。结果 VEGF-C和CCR7主要表达于卵巢癌细胞胞浆或/和胞膜内,VEGF-C和CCR7在淋巴结转移组的阳性表达率分别是71.7%和78.2%,在无淋巴结转移组的表达率分别是30.8%和26.9%,二者在淋巴结转移组的表达率均明显高于无淋巴结转移组(P<0.01)。VEGF-C和CCR7蛋白同时阳性表达在淋巴结转移组和非淋巴结转移组中的表达率分别为65.2%和15.4%,VEGF-C和CCR7的表达具有显著的相关性(P<0.01),联合检测VEGF-C和CCR7诊断卵巢癌淋巴结转移具有较高的准确度,ROC曲线下面积达0.791。结论 VEGF-C和CCR7表达在卵巢癌淋巴结转移过程中发挥重要作用,VEGF-C和CCR7在促进卵巢癌淋巴结转移中可能具有一定的协同作用,二者联合检测有助于预示卵巢癌淋巴结转移的判断。
Objective To investigate the effects of vascular endothelial growth factor（VEGF）-C and CCR7 on tumor lymph node metastasis,we observed the expression of VEGF-C and CCR7 in epithelial ovarian carcinoma. Methods Seventy-two paraffin-embedded stored specimens from patients with ovarian carcinoma were included in this study.They were devided into the lymph node metastasis group（n=46） and the nonmetastasis group（n=26）.The expressions of VEGF-C and CCR7 were detected by immunohistochemical staining. Results VEGF-C and CCR7 proteins were observed predominantly in the cytoplasm or/and on membrane of the tumor cells.The positive rate of VEGF-C and CCR7 expression in the lymph node metastasis group was 71.7% and 78.2% respectively,but 30.8% and 26.9% in the nonmetastasis group,with significant diferences（P0.01）.The synergic expression rate of VEGF-C and CCR7 was 65.2% and 15.4% in the lymph node metastasis group and the nonmetastasis group respectively,with statistical significance（P0.01）.High accuracy to predict lymph node metastasis was found in combined examination of VEGF-C and CCR7 expressions with an area of 0.791 under the ROC curve. Conclusion VEGF-C and CCR7 play an important role in the lymph node metastasis of ovarian carcinoma.VEGF-C and CCR7 act synergically in promoting tumor lymph node metastasis.Combined examination of VEGF-C and CCR7 might be beneficial for judging lymph node metastasis in ovarian carcinoma.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2011年第5期472-475,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
黑龙江省自然科学基金资助(D201041)
