摘要
目的探讨妊高征(PIH)大鼠颈交感神经干离断(TCST)对胎鼠脑的保护作用。方法妊娠 Wistar 大鼠32只,随机分为4组,每组8只,对照组(A 组)、PIH 组(B 组)、TCST 组(C 组)和假手术组(D 组)。B 组自孕14d 开始皮下注射 L-硝基精氨酸甲酯(L-NAME)12.5mg/100g,每天平均分两次注射,至孕20d,A 组给予等量生理盐水；C 组孕14d 行右 TCST,余同 B 组；D 组孕14d 行右颈交感神经干分离,但不离断,余同 B 组。各组于孕21d 剖宫取仔,观察胎鼠脑组织超微结构,测定脑组织 ATP、ADP、AMP 的含量、腺苷酸池(ATP+ADP+AMP)及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶活性。结果 A、C 组脑组织超微结构基本正常,B、D 组脑组织超微结构严重受损。与 A 组比较,B、D 组脑组织 ATP、ADP、AMP 的含量、腺苷酸池及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶的活性均降低；与 B 组比较,C 组脑组织 ATP、ADP、AMP 的含量、腺苷酸池及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶的活性均升高(P＜0.01)。结论 PIH 大鼠 TCST 对胎鼠脑有一定的保护作用,可能与改善脑细胞的能量代谢及相关的离子泵功能有关。
Objective To investigate the protective effect of transection of cervical sympathetic trunk （TCST） on the fetal brain in rats with pregnancy-induced hypertension （PIH）.Methods Thirty-two pregnant Wistar rats were randomly divided into 4 groups （ n = 8 each） ： A control group; B PIH group; C TCST ＋ PIH group and D sham operation ＋ PIH group. PIH was produced by L-NAME 12.5 mg· 100g^-1 given subcutaneously from 14^th-20^th day of gestation. In group A normal saline was given instead of L-NAME. In group C TCST was performed on the 14^th day of gestation and L-NAME was injected asin group B. In group D the cervical sympathetic trunk was only exposed but not cut. Caesarean section was performed and fetus was taken on the 21^th day of gestation. The uhrastructure of fetal brain was examined. The content of ATP, ADP and AMP in the fetal brain and the Na^＋-K^＋-ATPase and Ca^2＋-ATPase activity in neuronal plasmalemma were determined. Results The uhrastructure of the fetal brain was almost normal in group A and C, but was seriously damaged in group B and D.The ATP and adenylic acid content in the brain tissue and the Na^＋ -K^＋ -ATPase and Ca^2＋ -ATPase activity in neuronal plasmalemma were significantly lower in group B and D than in group A and C. Conclusion TCST has protective effect on the fetal brain in rats with PIH by improving energy supply and enhancing pump function of neuron.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第1期75-77,共3页
Chinese Journal of Anesthesiology
基金
辽宁省教委资助课题(20121270)
