摘要
目的探讨不完全性脊髓缺血损伤动物模型的建立方法，为不完全性脊髓缺血损伤机制研究提供理想的载体。方法24只新西兰大白兔按照随机数字表法分为对照组及3根组、4根组，每组8只。对照组用于排除麻醉和手术对运动诱发电位的影响；3根组、4根组分别结扎3根、4根腰动脉。各组麻醉后记录基线诱发电位，手术／结扎后30min、2d、7d记录诱发电位；麻醉清醒后、手术／结扎后2d、7d进行运动功能评分；手术／结扎后7d后取缺血中心区标本进行HE染色，镜下观察。结果3根组动物结扎后30min诱发电位波幅与对照组比较差异有统计学意义（P〈0．05），结扎后2d、7d与对照组比较差异无统计学意义（P〉0．051；4根组动物结扎后30min、2d、7d3个时间点诱发电位波幅与对照组比较差异均有统计学意义（P〈0．05）。3组动物手术／结扎后30min、2d、7d3个时间点的潜伏期与对照组比较差异均无统计学意义（P〉0．05）。各组动物运动功能评分结果与诱发电位波幅变化一致。结论结扎3根腰动脉可以造成可逆性不完全性脊髓缺血损伤，结扎4根腰动脉可以造成不可逆性不完全性脊髓缺血损伤。
Objective To explore the establishment of animal models of incomplete spinal cord ischemic injury to provide ideal carriers for researching the pathogenesis of incomplete spinal cord ischemic injury. Methods Twenty-four New Zealand rabbits were randomly divided into a control group （n=8） and 2 experimental groups （n=16）; control group only underwent sham-operation without inducing spinal cord injury to exclude the influences of anesthesia and surgery on motor evoked potential （MEP）; in the experimental groups, spinal cord ischemia injury models were established by the methods of selective ligation of segmental spinal artery from cranio-caudal direction. Baseline MEP after anesthetization were recorded, and the MEP 30 min, 2 and 7 d after vascular ligation were noted. Motor function was assessed after narcotic conscious, and 2 and 7 d after vascular ligation. The specimens 7 d after ligation were taken for HE staining. Results The amplitude of MEP in the experimental group having 3 lumbar artery ligation 30 minutes after ligation was significantly different as compared with that in the control group （P〈0.05）; no significant differences on the amplitude of MEP were noted between these 2 groups 2 and 7 d after ligation （/〉〉0.05）. Significant differences on the amplitude of MEP were noted between control group and experimental group having 4 lumbar artery ligation 30 min, and 2 and 7 d after ligation （P〈0.05）. The latency of all these rabbits showed no significant difference 30 min, and 2 and 7 d after ligation （P〉0.05）. The amplitude changes of MEP were accorded with the results of motor function scale. Conclusion Reversible incomplete spinal cord ischemia animal models can be established after 3 lumbar artery ligation; irreversible incomplete spinal cord ischemia animal models can be established after 4 lumbar artery ligation.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2011年第10期989-992,共4页
Chinese Journal of Neuromedicine
基金
福建省教育厅科技计划基金（JA08099）
关键词
脊髓损伤
动物模型
腰动脉
Spinal cord injury
Animal model
Lumbar
