摘要
目的探讨腺病毒介导的人骨形态发生蛋白-2(BMP-2)基因转染舌癌Tca8113细胞后,对Tca8113细胞形态和增殖活性的影响。方法分别以感染复数(MOI)为0、50、100的腺病毒为载体的BMP-2基因转染体外培养的舌癌Tca8113细胞。采用荧光倒置显微镜观察转染前后Tca8113细胞形态的变化,Western blot法检测Tca8113细胞BMP-2的表达,四甲基偶氮唑盐(MTT)法检测Tca8113细胞增殖能力,并绘制生长曲线。结果 MOI为100时转染率最高,转染前后Tca8113细胞形态变化不大,Western blot检测到Tca8113细胞BMP-2的表达,转染后Tca8113细胞的增殖活性降低。结论腺病毒介导的人BMP-2在体外能够抑制舌癌Tca8113细胞的增殖活性。
Objective To investigate the morphology and proliferation effects of adenovirus containing bone morpho-genetic protein-2（BMP-2） on tongue cancer Tca8113 cells.Methods Tca8113 cells were transfected with the AdBMP-2 of 0,50,100 multiplicity of infection（MOI） respectively.Inverted fluorescence microscope was used to evaluate the morphological changes of these cells.Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells.Methyl thiazolyl tetrazolium（MTT） assay was performed to monitor the proliferative activity of the infected Tca8113 cells and then the growth curve was made.Results The transfection efficiency rea-ched the highest when the MOI was 100.Moreover,the expression of BMP-2 was detected in Tca8113 cells by Western blot.There were no obvious morphological changes of the Tca8113 cells before and after transfection.And the prolifera-tion of transfected Tca8113 cells decreased compared with control.Conclusion Ad-BMP-2 gene can inhibit the pro-liferation of Tca8113 cells in vitro.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2011年第5期546-549,共4页
West China Journal of Stomatology
基金
山东省科学技术发展计划基金资助项目(2010GSF10239)
关键词
骨形态发生蛋白-2
腺病毒
基因转染
舌癌细胞
bone morphogenetic protein-2
adenovirus
gene transfection
tongue cancer cells
