摘要
中国的内脏利什曼病(也称为黑热病)传统上分为平原、山丘和荒漠三个不同类型的疫区,不同疫区的临床表现和流行病学特征有明显差异。这种差异是否可在不同疫区病原体分离株的分子水平上表现出来?如果是的话,这些分子水平的差异可否作为分子标记对不同疫区的分离株加以鉴别?本文总结了20年来本课题组通过动基体DNA、nDNA杂交、PCR-SSCP、RAPD及SSU rDNA可变区和LACK基因序列测定等方法进行的技术,结果显示:来自中国不同疫区的利什曼原虫分离株在分子水平上存在异源性,为不同类型利什曼病的防治对策提供理论依据。
Leishmaniasis(Kala-azar)from different endemic regions of China expresses different clinic and epidemiological features,and traditionally is classified as hilly,plain and desert types/foci.We concentrated our review on whether the pathogens from those foci were different at molecular level,if so,whether there are were molecular markers readily identifiable by molecular technologies.This was a review of a 20-year search for such markers by u-sing kinetoplastic DNA(kDNA),nDNA hybridization,PCR-SSCP,RAPD and sequence analysis of SSU rDNA vari-able regions and LACK gene.The results showed that heterogeneities at molecular level exist in Leishmania isolated from different foci of China,which could be used as markers for different types of Leishmaniasis in China.
作者
马莹
卜玲毅
胡孝素
Ma Ying;Bu Lingyi;Hu Xiaosu(Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;Department of Microbiology and Parasitology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and School of Basic Medicine,Peking Union Medical College,Beijing 100005,China;Department of Parasitology,School of Preclinical and Forensic Medicine,Sichnan University,Chengdu 610041,China)
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2011年第5期997-1000,共4页
Journal of Biomedical Engineering
