摘要
[目的]建立了酶催化动力学光度法测定盐酸氯丙嗪的新方法。[方法]在pH 9.8的NH3-NH4Cl缓冲溶液中,利用盐酸氯丙嗪对牛血红蛋白(Hemoglobin,Hb)模拟酶催化体系的抑制作用,研究了该抑制反应的最佳试验条件及动力学行为。[结果]测定的线性范围为0.25～25.00μg/ml,方法检出限为0.026μg/ml。对浓度为2.5μg/ml的盐酸氯丙嗪进行11次平行测定,其相对标准偏差为4.5%。[结论]该方法简单、快速、灵敏,可用于动物性食品中盐酸氯丙嗪残留的检测。
[Objective] To develop a high sensitive and simple spectrophotometric method for the determination of chloropromazine hydrochloride.[Method] The method is based on the inhibition of hemoglobin for the oxidation of H2O2 with acid chrome blue K in the pH 9.8 NH3-NH4Cl buffer.The percentage inhibition of system is calculated under the optimal experimental condition.[Results] The calibration curve is linear in the range of 0.25-25.00 μg/ml,with the detection limit of 0.026 μg/ml.The relative standard deviation of this method is 4.5%at 2.5 μg/ml for 11 determination.[Conclusion] The method is sample,rapid,sensitive and can be used for the determination of chloropromazine hydrochloride residue in animal-derived food with satisfactory result.
出处
《安徽农业科学》
CAS
北大核心
2011年第29期18240-18240,18261,共2页
Journal of Anhui Agricultural Sciences
基金
河南省科技厅基础与前沿技术研究项目(112300410013)
关键词
盐酸氯丙嗪残留
酶催化光度法
血红蛋白
Chloropromazine hydrochloride residue
Enzymatic catalytic spectrophotometry
Hemoglobin
