摘要
目的观察小半夏加茯苓汤(Xiaobanxia Fuling Decoction,XBFD)对顺铂(cisplatin,DDP)诱发的化疗性大鼠异食癖的防治作用,并探讨其作用机制。方法雄性SD大鼠42只,随机分为空白组、模型组、XBFD高、中、低剂量[30、15、7.5g/(kg.d)]组、阿瑞吡坦[aprepitant,13mg/(kg.d)]组及纯中药[XBFD15g/(kg.d)]组,除空白组和纯中药组外,其余各组大鼠于实验第3天腹腔注射DDP[3mg/(kg.d)],建立化疗性大鼠异食癖模型;观察XBFD对大鼠异食高岭土量、摄食试料量及一般情况的影响;在实验第6天即DDP化疗72h后,分别用免疫组化法和实时荧光定量PCR法检测大鼠延髓及胃窦NK1受体(neurokinin 1 receptor,NK1R)蛋白和mRNA表达。结果实验第3～5天,与空白组比较,各组大鼠异食高岭土量明显增加(P<0.01);与模型组比较,XBFD各剂量组大鼠异食高岭土量均有减少(P<0.05),XBFD高剂量组大鼠实验第3天摄食试料量增加(P<0.05),XBFD高、中剂量组大鼠延髓和胃窦NK1R蛋白和mRNA的表达量降低(P<0.05)。结论 XBFD可防治DDP诱发的大鼠异食癖;此作用可能与降低延髓和胃窦NK1R的表达有关。
Objective To investigate the preventive and therapeutic effects of Xiaobanxia Fuling Decoction（XBFD） on cisplatin-induced pica rats and to study its mechanism.Methods Forty-two male Sprague-Dawley rats were randomly divided into the following 7 groups,i.e.,the blank control group,the model group,the high-,middle-,and low-dose XBFD groups（at the daily dose of 30,15,and 7.5 g/kg,respectively）,the aprepitant（at the daily dose of 13 mg/kg）,and pure Chinese medicine group（at the daily dose of XBFD 15 g/kg）,6 in each group.On the 3rd day of this study,3 mg/kg cisplatin was intraperitoneally injected to rats except the blank control group and the model group to establish the pica rat model.The consumptions of kaolin,food,and the general situation of rats were observed.The protein and mRNA expressions of neurokinin 1 receptor（NK1R） in both the medulla oblongata and the gastric antrum were measured by immunohistochemical assay and Real-time fluorescent quantitative PCR respectively on the sixth day of this study.Results On the third,fourth,and fifth day of this study,the consumption of kaolin of rats significantly increased when compared with the blank control group（P0.01）.Compared with the model group,the consumption of kaolin significantly decreased in the high-,middle-,and low-dose XBFD groups on the third,fourth,and fifth day of this study（P0.05）.The food intake of rats in the high-dose XBFD groups significantly increased when compared with the model group on the third day of this study（P0.05）.The protein and mRNA expressions of NK1R in the medulla oblongata and the gastric antrum significantly decreased in the high-and middle-dose XBFD groups when compared with the model group（P0.05）.Conclusions XBFD could prevent and treat cisplatin-induced pica in rats.Its effect might be correlated with decreasing expressions of NK1R in the medulla oblongata and the gastric antrum.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2011年第10期1414-1418,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
贵州省中医药管理局资助课题(No.D288)
