摘要
目的:观察呕必宁对顺铂诱发大鼠胃、延髓组织SP含量及NK1受体表达改变的影响,探讨呕必宁防治顺铂诱发呕吐机制同时,为中医学"胃气上逆"等理论提供实验依据。方法:Wistar大鼠54只,分成对照组、模型组和中药组,分别灌服盐水、呕必宁煎液6天时,对照组腹腔注射盐水,余两组注射顺铂48h后,取延髓、胃组织,放免法检测SP含量,免疫组化法检测NK1表达,并进行灰度值测定。结果:模型组大鼠胃窦及延髓组织SP含量、NK1表达高于对照组和中药组(P(0.01);中药组SP含量及NK1表达与对照组无明显差异(P(0.05)。结论:呕必宁能够抑制顺铂所致大鼠胃、延髓组织SP升高及NK1表达。大鼠胃及延髓组织SP增多、NK1表达上调可能是"胃气上逆"的病理学基础之一。
Objective ： In order to detect the mechanism of Oubining preventing and treating vomiting induced by cisplatin （DDP） and provide experimental basis for the ＂ adverse ascending of Stomach - Qi＂ , action of Oubining to the changes of substance P （SP） concentration and neurokinin receptor 1 （NK1 -R） in rats＇stomach and medullar tissues induced by DDP. Methods .. 54 Wistar rats were randomly divided into three groups： control group, model group and Chinese herbal group, and the rats in later groups were given saline and oubining via intragastric administration. 6 days later, the rats in control group were given DDP and rats in other groups were given saline via intraperitoneal injection. 48 hours later medullar tissue and gastric antrum were taken out to measure SP levels with radioimmunoassay and detect NK1 -R expression by immunohistochemistry technique. Results ： SP concentration and NK1 - R expression in the tissues from gastric antrum and medulla oblongata in the model group were higher than those in the control group and Chinese herbal group （ P 〈 0.01 ）, and there is no significance of SP concentrations and NK1 - R expression between the Chinese herbal group and the control group （ P 〉 0. 05）. Conclusion ： Oubining can inhibit the SP level increase and strong expression of NK1 - R in medulla oblongata and gastric antrum induced by DDP. And the SP level increase and strong expression of NK1 - R in medulla oblongata and gastric antrum might be the pathologic basis of ＂adverse ascending of Stomach - Qi＂.
出处
《中华中医药学刊》
CAS
2008年第3期466-468,共3页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅基金资助项目(20040228)
