摘要
目的监测河南省肠出血性大肠埃希菌O157∶H7在腹泻患者和宿主动物中带菌及毒力基因情况,探索疾病发生的原因和相关因素。方法对2005-2009年河南省监测点所送可疑菌株进行血清学和PCR复核,对确认菌株进行stx1、stx2、eaeA、hlyA毒力基因测定。结果河南省2005-2009年共监测各类标本10 732份,检出O157∶H7菌株255株,检出率为2.38%;其中动物粪便标本的检出率为6.31%,检出率最高的为羊粪(8.04%),其次为牛粪(7.20%);各年份菌株检出率之间有一定差异;河南省肠出血性大肠埃希菌O157∶H7的产毒株主要来自于羊、牛、鸡粪便及蝇类和生肉标本;毒株类型主要为stx2、eaeA、hlyA组合型。结论河南省肠出血性大肠埃希菌O157∶H7在人群和各类动物中均存在,最重要的动物宿主是羊和牛;部分食品在加工环节有可能被污染,存在引起暴发的危险性。
Objective To determine the presence and virulence genes of enterohemorrhagic Escherichia coli（EHEC）O157∶H7 in diarrhea patients and host animals in Henan province in order to identify the cause and related risk factors.Methods Suspicious samples collected at surveillance sites from 2005 to 2009 were subjected to serological detection and PCR,and tested for stx1,stx2,eaeA,hlyA virulence genes.Results Of the 10 732 serum samples,255（2.38%） were positive for O157∶H7 strains.The detection rate in animal stool samples was 6.31%（8.04% for sheep and 7.20% for cows）.Different detection rates were observed in different years.Most EHEC O157∶H7 strains were from sheep,cattle and chicken feces.A combination of virulence genes stx2,eaeA,hlyA was predominant.Conclusion EHEC O157∶H7 existed in the population and various animals in Henan province.The most important animal hosts were sheep and cows.Contaminated food during processing was a possible cause and might lead to potential outbreaks.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2011年第5期427-428,435,共3页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项课题(2009ZX10004-203)~~
