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TRAIL诱导胃癌细胞凋亡的实验研究

Experimental studies of TRAIL protein on apoptosis of tumor cell
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摘要 目的研究TRAIL对胃癌细胞株MKN28细胞体外诱导凋亡的作用及机制,并研究此过程中Caspase-3的表达规律及与凋亡效应的相关性。方法培养细胞,MTT法分析细胞增殖抑制率及细胞存活率,流式细胞仪分析细胞凋亡及细胞周期,细胞免疫组化检测细胞Caspase-3的表达水平,AO/EB染色观察凋亡细胞。结果 TRAIL可明显抑制MKN28细胞的增殖,并可诱导细胞的凋亡。细胞阻滞于G0/G1期,Caspase-3在MKN28细胞中低表达,经TRAIL处理细胞后高表达。AO/EB染色后可见典型细胞凋亡特征。结论 TRAIL可诱导MKN28细胞株的凋亡,并呈剂量依赖性。TRAIL通过上调Caspase-3的表达,阻滞细胞周期,诱导MKN28细胞的凋亡。 Objective：To investigate apoptosis induction of TRAIL and its mechanism in gastric cancer cell MKN28 line in vitro,and to study the express of Caspase-3 and its correlation with apoptosis.Methods： Their influence on cell proliferative activity was detected by MTT assay.The cell apoptosis and cell cycle was determined by flow cytometric（FCM） analysis.The expression of Caspase-3 was detected by immunohistochemistry.apoptosis was detected by AO/EB staining.Results： TRAIL could obviously inhibit the proliferation of MKN28 cells,and induce its apoptosis.Cell cycle was arrested in the G0/G1 phase.After treatment of TRAIL,expression of Caspase-3 was induced in MKN28 cells.MKN28 cells showed the typical features of apoptosis after AO/EB staining.Conclusion： TRAIL had effects of inducing apoptosis on MKN28 cell lines with a dose-dependence.The apoptosis of MKN28 cells was induced by TRAIL through inducing expression of Caspase-3 and arresting cell cycle.
作者 张玲 王庆才
机构地区 泰山医学院附属泰山医院肿瘤内科 泰山医学院附属泰山医院消化内科
出处 《泰山医学院学报》 CAS 2011年第8期561-564,共4页 Journal of Taishan Medical College
关键词 TRAIL 凋亡 MKN28细胞 CASPASE-3 TRAIL apoptosis cell MKN28 Caspase-3
分类号 R735.2 [医药卫生—肿瘤]
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参考文献9

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二级参考文献8

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泰山医学院学报
2011年 第8期

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