摘要
目的探讨华蟾素注射液对人肝癌HepG-2细胞增殖及周期的影响。方法应用噻唑蓝还原法(MTT)检测华蟾素注射液对HepG-2细胞增殖的影响;应用流式细胞术(FCM)检测HepG-2细胞周期分布;采用逆转录-多聚酶链反应技术(RT-PCR)检测华蟾素注射液对HepG-2细胞CyclinA和CDK2 mRNA表达水平的影响;应用比色定量法检测华蟾素注射液对HepG-2细胞内CDK2及CyclinA活性的影响。结果华蟾素注射液可以抑制HepG-2细胞增殖,其抑制作用具有时间和剂量依赖性;可将HepG2细胞阻滞于S期;抑制HepG-2细胞CDK2、CyclinAmRNA水平表达;使HepG-2细胞CyclinA、CDK2活性降低。结论华蟾素注射液能够抑制人肝癌HepG-2细胞增殖,并将细胞阻滞于S期,诱导细胞凋亡,机制可能是通过影响CyclinA、CDK2活性,下调CDK2、CyclinAmRNA水平表达实现的。
Objective To study the effect of cinobufacin injection on the proliferation and cell cycle of human hepatoma HepG-2 cell. Methods Cell proliferation was analysed by MTT assay; Flow cytometry （FCM） was emplayed to detect tumor cell cycle distribution; Expression of CyclinA, CDK2 mRNA levels in HepG-2 cells was analysed by PT-PCR;Quantitative colorimetric assay was used to analyse cyclinA/CDK2 activity in HepG-2 cells .Results Cinobufacini injection significantly inhibited HepG-2 cells proliferation in doses-and time-dependent ways;FCM analysis expressed cinobufacin injection induced cell cycle arrest at S phase ;PT-PCR assay showed cinobufacin injection down-regulated CDK2, CyclinA expression at mRNA levels; Quantitative colorimetric assay displayed cinobufacin injection deceased cyclinA/CDK2 activity in HepG-2 cells.Conclusion Human hepotocarcinoma HepG-2 cell growth can be inhibited and cell cycle can be induced to arrest at S phase through injection.Conclusion The mechanism might be partly related to the down-regulation of CDK2, CyclinA expression and inhibition of cyclinA/CDK2 activity .
出处
《中国医药指南》
2011年第28期206-208,共3页
Guide of China Medicine
