摘要
研究丝瓜过氧化物酶最适温度、最适pH值、Km、Vmax及最适底物,同时通过正交试验探讨不同抑制剂组合对丝瓜过氧化物酶的抑制作用。结果表明,丝瓜过氧化物酶最适温度为45℃,最适pH值为5.0,以邻苯三酚为最适底物,Km=0.001516mol/L,Vmax=440.7U(/min.g)。正交试验结果表明,最佳抑制剂组合为6mmol/L柠檬酸+6mmol/L EDTA+6mmol/L半胱氨酸+6mmol/L VC。在此条件下抑制率为58.89%。
The optimum temperature, pH value, Km Vmax and optimum substrate of peroxidase （POD） from Luffa cylindrica （Lima.） Roem were studied, and the effects of different inhibitors combination on peroxidase activity were also explored. The results shown that the optimum temperature of POD was 45℃, pH value 5.0, Kin=0.001516mol/L and Vmax=440.7U （min·g） using pyrogallol as optimum substrate. Orthogonal experimental results showed that the optimization inhibitors combination were as follows： 6mmol/L citric acid＋6mmol/L EDTA＋6mmol/L L-cys＋6mmol/L VC. Under these conditions, inhibition ratio was 58.89%.
出处
《中国酿造》
CAS
北大核心
2011年第10期81-85,共5页
China Brewing
基金
地震灾后新农村建设技术集成与示范(2008BAR51B05)
关键词
丝瓜
过氧化物酶
特性
褐变
Luffa cylindrica （Linn）Roem
peroxidase
characteristics
browning
