摘要
以西伯利亚远志带腋芽的茎段为外植体,探讨不同激素处理6-BAI、BA、NAA组合对西伯利亚远志试管苗增殖及生根的影响,建立一套西伯利亚远志离体培养技术体系。结果表明:适合西伯利亚远志增殖培养基为MS+6-BA 0.5 mg/L+IBA 0.1 mg/L,生根培养基为1/2MS+IBA 1.0 mg/L,在此体系中增殖系数达3.15,生根率100%,移栽成活率可达100%。
Stems with axillaries bud of Polygala sibirica L.were used as explants,and placed on MS culture medium with different hormone of 6-BA,IBA,NAA combination and different concentrations,and established a new technical system of plantlet regeneration and rapid propagation.The results showed that the optimum multiplication culture medium was MS＋6-BA 0.5 mg/L＋IBA 0.1 mg/L;the rooting culture medium was 1/2MS＋IBA 1.0 mg/L.In this system,the highest propagation coefficient was 3.15,the rooting rate was 100%,and the survived plantlets rate of transplant was 100%.
出处
《北方园艺》
CAS
北大核心
2011年第12期106-109,共4页
Northern Horticulture
关键词
西伯利亚远志
组织培养
快速繁殖
Polygala sibirica L.
tissue culture
rapid propagation
