摘要
以新西兰麻种子为外植体材料,进行组培试验,建立离体培养技术体系。结果表明,以75%酒精消毒60 s,再用0.1%Hg Cl2消毒5 min的效果最好;丛生芽增殖的适宜培养基为MS+6-BA 2 mg·L^(-1)+KT 0.4 mg·L^(-1);诱导不定根的适宜培养基为MS+IBA 1 mg·L^(-1)+NAA 0.5 mg·L^(-1);生根组培苗炼苗移栽30 d后,成活率达84%以上。
The study used Phormium tenax Forst＇s seeds as the explants, conducted the research of tissue cuhure techniques ,and established the rapid propagation system in vitro. The results indicated that using 75% Alcohol to disinfection for 60 s and 0. 1% HgCl2 to disinfection for 5 min were most effective;MS ＋6-BA 2mg · L^-1 ＋ KT 0. 4 mg · L^-1 was the optimizing medium for multi- propagation ; MS ＋ IBA 1 mg· L^-1 ＋ NAA 0. 5 mg· L^-1 was the best medium for rooting; After 30 days transplanted into greenhouse, the survival rate of young plants were over 84%.
作者
毕玮
吴涛
耿云芬
原晓龙
王娟
BI Wei WU Tao GENG Yun-fen YUAN Xiao-long WANG Juan(Yunnan Academy of Forestry, Kunming 650201, Yunnan, China Yunnan Laboratory for Conservation of Rare ,Endangered ＆ Endemic Forest Plants ,Public Key Laboratory of the State Forestry Administration , Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,China-New Zealand joint Laboratory of Cultivation and Exploitation of Forest Plants, Kunming 650201, Yunnan, China)
出处
《福建林业科技》
2016年第3期178-182,共5页
Journal of Fujian Forestry Science and Technology
基金
云南省应用基础研究重点项目(2013FA054)
云南省科技计划-对外科技合作计划项目(2014IA013)
云南省中青年学术技术带头人后备人才培养项目(2010CI016)
关键词
新西兰麻
组织培养
消毒方法
培养基
Phormium tenax Forst
tissue culture
disinfection method
culture medium
