摘要
[目的]了解除草剂对黑麦幼苗早期生长的影响。[方法]从生理生化和细胞水平分析了2种除草剂[阿特拉津(Atrazine)和甲酰胺草磷(Amiprophos methyl,APM)]对黑麦早期幼苗生长的影响。[结果]Atrazine可明显降低黑麦幼苗叶绿素a、b和可溶性蛋白质的含量。当用0.100和1.000 mg/LAtrazine处理黑麦幼苗16h时,叶绿素a、b含量分别从对照的1.26(a)、0.49(b) mg/g(FW)下降至1.15(a)、0.46(b) mg/g(FW)和0.81(a)、0.33(b) mg/g(FW)。可溶性蛋白质含量则随着Atrazine处理浓度的增加而减少。Atrazine对黑麦分生组织细胞有丝分裂和染色体结构无显著影响。APM处理后,黑麦幼苗叶绿素a、b和可溶性蛋白质含量无显著变化,但分生组织细胞有丝分裂和染色体结构变异如桥、多极分裂、滞后染色体和不均等分裂中期细胞数量显著增加。[结论]黑麦中Atrazine使用的临界剂量为0.100～1.000 mg/L,而APM为4.000mg/L。
[Objective] The aim was to study the effect of herbicide on the growth of early seedlings of rye（Secale cereale）.[Method] Effect of two kinds of herbicide（Atrazine and APM） on seedling growth of rye was investigated at the physiological,biochemical and cellular level.[Result] The Atrazin significantly decreased the contents of chlorophyll a,b and soluble proteins.Rye seeds were treated with 0.100 and 1.000 mg/L Atrazine for 16 h,the contents of chlorophyll a and b decreased from 1.26（a）,0.49（b） mg/g FW（control） to 1.15（a）,0.46（b） mg/g FW（0.100 mg/L） and 0.81（a）,0.33（b） mg/g FW（1.000 mg/L）.The content of soluble protein decreased with the increasing concentration of Atrazin.Atrazin had no significant influence on the cell division and chromosome structure variation.The contents of chlorophyll a,b and soluble proteins had no significantly change under the treatment of APM,but the number of chromosome structure variation such as chromosome bridge,multipolar division cells,lagging chromosome and unequal division cells increased significantly.[Conclusion] The critical concentration of Atrazine was 0.100-1.000 and 4.000 mg/L of APM in rye.
出处
《安徽农业科学》
CAS
北大核心
2011年第28期17293-17295,共3页
Journal of Anhui Agricultural Sciences
基金
教育部科学研究重点项目(02010)
关键词
除草剂
黑麦
叶绿素含量
可溶性蛋白质
染色体结构变异
Herbicides
Secale cereal
Chlorophyll content
Soluble protein
Chromosome structure variation
