摘要
目的通过检测错配修复基因hMLH1和hMSH2在宫颈病变组织中的表达,探讨其在该病进展中的作用及意义。方法采用免疫组化SP法对119例患者宫颈组织中hMLH1和hMSH2的表达进行检测,应用统计学分析软件SPSS8.1对资料数据进行统计学分析。结果错配修复基因hMLH1在慢性宫颈炎、宫颈上皮内瘤样变CINI、CINⅡ～Ⅲ、宫颈癌中的阴性表达率分别为18.8%、20%、48.4%、53.8%,错配修复基因hMSH2分别为12.5%、10.0%、41.9%、38.5%。二者阴性表达率均随宫颈病变程度的增加而升高。宫颈各级病变中hMLH1和hMSH2阴性表达率差异均有统计学意义(P<0.05)。结论 hMLH1、hMSH2基因的缺陷是宫颈癌发生、发展的重要机制。
Objective To explore the importance of the expression of hMLH1和 and hMSH2 in etiological mechanism of cervical intraepithelial neoplasia lesions by analyzing.Methods Immunohistochemical staining with SP were performed to analysis the gene expression of hMLH1 and hMSH2.All statistical analyses were conducted by using SPSS 14.0 software package.Results The expression rate of hMLH1 in the cervicitis,cervicalintraepithelial neoplasia1（CINⅠ） and CINⅡ-Ⅲ、uterine cervix cancer patients was 18.8%,20%,48.4%,53.8%,and the expression rate of hMSH2 was 12.5%,10.0%,41.9%,38.5%.Conclusion Our results suggests that the the mismatch repair gene（hMLH1 and hMSH2） are intensively correlated with prognosis of the cervical pathological changes,especial with cervical cancer.
出处
《中外医学研究》
2011年第29期15-16,共2页
CHINESE AND FOREIGN MEDICAL RESEARCH
