摘要
目的了解广东省台山市梅毒感染情况,对梅毒检测方法学进行评价,为制订相应的干预和实验室检测策略提供依据。方法采用快速血浆反应素试验(RPR)和梅毒螺旋体酶联免疫吸附试验(TP-ELISA)方法进行检测,阳性结果均用梅毒螺旋体明胶凝集试验(TPPA)进行确认。结果 2010年共596份血清样本(上半年319份,下半年277份),在TPPA法确诊的45份阳性标本中,RPR法检出阳性16份,敏感性为35.56%;ELISA法检出阳性43份,敏感性为95.56%。RPR法共检出阳性21份,有5份确诊为阴性,特异性为99.16%;ELISA法共检出阳性46份,有3例确诊为阴性,特异性为99.50%。RPR和TPPA阳性结果差异有统计学意义(P<0.05),ELISA与TPPA阳性结果差异无统计学意义(P>0.05)。结论 ELISA检测法比RPR检测法的敏感性高,特异性好,可作为梅毒感染的一种理想检测方法,对控制梅毒的性传播有重要意义。但对ELISA阳性标本应做TPPA确认。
Objective To assess the syphilis infection situation,and to evaluate syphilis testing methods and provide an appropriate intervention and laboratory testing strategy.Methods We took rapid plasma reagin（RPR） and enzyme-linked immunosorbent assay（ELISA） as the testing methods and positive results were confirmed with treponema pallidum agglutination test（TPPA）.Results There were 596 serum samples this year（319 in the first half year and 277 in the rest）.Among the 45 TPPA positive samples,16 samples turned out to be positive under the method of RPR with a sensitivity rate of 35.56%.While using ELISA there were 43 positive cases with a sensitivity rate of 95.56%.Among the 21 RPR positive samples,3 were confirmed as negative,with specificity of 99.16%.While among the 46 ELISA positive samples,5 were confirmed negative,with specificity of 99.50%.There was a significant difference between the positive results of RPR and TPPA（P〈0.05）,but not between ELISA and TPPA（P〈0.05）.Conclusion ELISA testing measurement is more advantageous than RPR with its higher sensitivity and better sensitivity,so it can be an ideal measurement method for syphilis detection and important for controlling syphilis by sex.However,a confirmative test should be made to ELISA positive samples by TPPA.
出处
《检验医学与临床》
CAS
2011年第20期2470-2471,共2页
Laboratory Medicine and Clinic
