摘要
目的调查医院耐碳青霉烯类鲍氏不动杆菌(CRAB)的同源性,并了解是否存在耐药菌株的克隆流行。方法收集2009年6月-2010年6月临床分离的CRAB 39株,用微量稀释法检测其对13种抗菌药物的敏感性;用脉冲场凝胶电泳(PFGE)的方法对所分离的菌株进行分子分型。结果 39株鲍氏不动杆菌主要分离自ICU;对碳青霉烯类抗菌药物的耐药率为100.0%,敏感率较好的为米诺环素、阿米卡星、多黏菌素E,敏感率分别为100.0%、94.9%、61.5%;脉冲凝胶电泳结果显示,39株CRAB分为A、B、C型,其中A型17株、A1亚型1株、A2亚型1株、A3亚型1株,B型15株、B1亚型2株、B2亚型1株,C型1株。结论调查期间医院主要为A型和B型菌株克隆流行,C型菌株为散发株;菌株流行主要集中在ICU。
OBJECTIVE To study the homology of CRAB and to identify the prevalence of the drug-resistant clones.METHODS A total of 39 consecutive CRAB isolates collected from June 2009 to June 2010 were screened,microdilution method was used to determine the minimal inhibitory concentration（MICs） and pulsed-field gel electrophoresis（PFGE） was used to detect homology of Acinetobacter baumannii.RESULTS Totally 39 isolates were mainly come from ICU.Resistant rates of imipenem and meropenem were 100.0%.Sensitivity rates of minocycline,amikacin and polymyxin were 100.0%,94.9% and 61.5%,respectively.All of 39 isolates belonged to three PFGE pulsotypes（A,B,C）.Pulsotype A were 20 isolates（17 of type A,1 of subtype A1,1 of subtype A2,1 of subtype A3）;Pulsotype B were 18 isolates（15 of type B,2 of subtype B1,1 of subtype B2）;1 pulsotype C isolate.CONCLUSION The emergence of CRAB is associated with the spread of A.baumannii strains of clone A and B.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第20期4198-4201,共4页
Chinese Journal of Nosocomiology
