摘要
目的研究成都地区献血者人群HCV感染的基因型分布。方法从血液筛查出的抗-HCV反应性血清标本中提取HCV RNA,RT-PCR获得HCV核酸C区及NS5B区序列片段,用MEGA软件构建序列进化树,分析HCV的亚型分布特征及可能的原因。结果对313例HCV RNA阳性标本分型结果如下,1b亚型283例,2a亚型14例,3b亚型7例,3a亚型3例,6a亚型5例,6u亚型1例;4和5基因型没有发现。结论在成都地区献血者人群感染的HCV中共有6个亚型分布。其中1b是绝对优势亚型,占90.41%(283/313);6u亚型在成都地区首次被检测到。
Objective To study the HCV distribution characterization of genotypes from volunteer blood donors in Chengdu. Methods HCV RNA was extracted from samples with positive anti-HCV and HCV fragments and was amplified u- sing RT-PCR. Purified productions of PCR were sequenced and phylogenetic trees were constructed based on the HCV NS5B regions from 313 sequences. Results HCV genotypes were determined with the following results： lb in 283, 2a in 14, 3b in 7, 3a in 3, 6a in 5 and 6u in l. Genotype 4 and 5 were undetected. Conclusion Six HCV subtypes were found in blood do- nors from Chengdu area. Subtype lb was the most common and accounted for approximately 90. 41% （283/313）, and one subtvoe 6u was firstly isolated from Chengdu area.
出处
《中国输血杂志》
CAS
北大核心
2016年第12期1380-1384,共5页
Chinese Journal of Blood Transfusion
基金
四川省卫计委科研项目(201215)
关键词
HCV
基因型
分布
HCV
genotypes
distribution
