摘要
目的:建立以细胞凋亡信号-磷脂酰丝氨酸(PS)介导的小胶质细胞体外吞噬模型,研究小胶质细胞发挥吞噬功能时炎症因子的表达变化。方法:用N-乙基马来酰胺(NEM)预处理红细胞,随后整合氧化PS,制备含氧化PS信号的红细胞凋亡模型,并测定小胶质细胞对整合氧化PS信号红细胞吞噬率的变化。同时,real-time PCR检测小胶质细胞中炎症因子白细胞介素1β(IL-1β)和肿瘤坏死因子(TNF-α)的表达情况,研究小胶质细胞吞噬功能与炎症功能之间的关系。结果:小胶质细胞对整合氧化PS红细胞的吞噬率显著高于对照组(P<0.01),同时炎症因子IL-1β和TNF-α的mRNA表达水平明显减少。结论:成功制备小胶质细胞体外吞噬凋亡细胞模型,小胶质细胞的吞噬作用可能是自身炎症因子IL-1β和TNF-α在转录水平上降低的诱因。
AIM： To establish a phagocytosis model of microglia mediated by apoptotic signal phosphatidylserine（PS） and to explore the expression of inflammatory cytokines in the phagocytic process of microglia.METHODS： The apoptosis model of red blood cells（RBC） was established by incubating RBC with oxidized PS（PSox） liposomes after pretreatment of RBC with N-ethylmaleimide（NEM）.The phagocytic rate of microglia to RBC integrated with oxidation of PS signal was measured.Real-time PCR was used to detect the expression of inflammatory cytokines such as interleukin-1 beta（IL-1β） and tumor necrosis factor alpha（TNF-α） in microglia.RESULTS： RBC incubated with PSox liposomes significantly enhanced the phagocytic rate of microglia.Inflammatory cytokines such as IL-1β and TNF-α were apparently decreased at the transcription level when microglia exerted phagocytosis on RBC.CONCLUSION： The in vitro phagocytosis model of microglia was established by integrating PSox with RBC directly.The microglia decreases the expression of inflammatory cytokines at the transcription level in the phagocytic process when RBC were integrated with oxidation of PS signal.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第9期1848-1851,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30700931No.30970672)
