摘要
目的探讨不同浓度的大蒜油对日本血吸虫尾蚴活性的影响。方法采用蒸馏法提取大蒜油,并配制成不同浓度,观察杀灭日本血吸虫尾蚴的时间;用经大蒜油处理3min的日本血吸虫尾蚴感染小鼠,45d后处死,计数检获成虫数,计算感染率和减虫率,试验设去氯离子自来水为对照。结果大蒜油原液1s内可迅速杀死日本血吸虫尾蚴,稀释度为10-2、10-3、10-4、10-5、10-6的大蒜油杀死日本血吸虫尾蚴的平均时间分别为(2.4±0.54)s、(28.4±4.24)s、(60.6±3.43)s、(387.0±19.05)s和(820.4±22.34)s;用以上稀释度大蒜油处理后的血吸虫尾蚴感染小鼠,感染率分别为0、0、0、0、60.00%、80.00%,对照组感染率为100%。结论大蒜油有较强的杀灭日本血吸虫尾蚴作用。
Objective To examine the anti-cercarial activity of garlic oil in different concentrations.Methods Garlic oil was extracted using distillation and prepared in different concentrations,the time different concentrations of garlic oil took to kill cercariae of Schistosoma japonicum was measured.Mice infected with the cercariae were treated with different concentrations of garlic oil in for 3 minutes.Forty-five days later,the mice were sacrificed and adult S.japonicum were counted.The infection rate and the worm reduction rate were calculated.A control group was given chloride-ion-free water.Results The stock solution of garlic oil killed the cercariae of S.japonicum in 1 s.A concentration of 10-2 killed cercariae in an average time of（2.4±0.54） s,a concentration of 10-3 did so in（28.4±4.24） s,a concentration of 10-4 did so in（60.6±3.43） s,a concentration of 10-5 did so in（387.0±19.05） s,and a concentration of 10-6 did so in（820.4±22.34） s.Different concentrations resulted in infection rates of 0,0,0,0,60.00%,and 80.00%.The rate of infection for the control group was 100%.Conclusion Garlic oil has the ability to kill the cercariae of S.japonicum.
出处
《中国病原生物学杂志》
CSCD
2011年第9期666-667,共2页
Journal of Pathogen Biology
基金
湖北省教育厅资助项目(No.B20091301)
宜昌市科技局资助项目(No.A08302-28)
三峡大学人才启动基金(No.KJ2008B097)
关键词
大蒜油
血吸虫
日本
尾蚴
Garlic oil
extraction
Schistosoma japonicum
anticercaria activity
