摘要
本文以液体闪烁谱仪计数法,测定离体缺血再灌注鼠心肌线粒体内^(45)Ca^(2+)的放射强度(cpm),观察ATP-MgCl_2、超氧化物歧化酶(SOD)和异搏定对缺血再灌注鼠心肌线粒体内钙离子的影响。结果表明,ATP-MgCl_2,SOD和异搏定组的^(45)Ca^(2+)cpm显著低于对照组的^(45)Ca^(2+)cpm(P<0.01),ATP-MgCl_2,组和SOD组的^(45)Ca^2+cpm又低于异搏定组的^(45)Ca^(2+)cpm,而ATP-MgCl_2组和SOD组之间无显著性差异(P>0.05),结果提示,ATP-MgCl_2、SOD和异搏定三种药物均能阻止离体大鼠心肌线柱体内Ca^(2+)积聚,从而保护心肌细胞缺血再灌注性损伤。
(45)~Ca^(2+) radiation intensity (cpm)was measured using liquid scintillation counting method, and the effects of ATP-MgCl_2, superoxide dismutase(SOD)and verapamil om mitochondrial (45)~Ca^(2+) in isolated myocardium of the rat with experimental ischemia reperfusion were studied respectively. It demonstrates that the (45)~Ca^(2+) cpms in ATP-MgCl_2, SOD, and verapamil groups were significantly weaker than that in control groups, and the cpms in ATP-MgCl_2 and SOD groups were weaker than that in verapamil group. The results suggest that ATP-MgCl_2, SOD, and verapamil can inhibit the accumulation of mitochondrial (45)~Ca^(2+) in myocardium induced by ischemia-reperfusion; these effects of ATPMgCl_2 and SOD are superior to that of verapamil.
出处
《延边医学院学报》
1990年第4期245-248,共4页
Journal of Medical Science Yanbian University
关键词
缺血
线粒体
心肌
钙
异搏定
镁
myocardium
mitochondria
ischcmia
perfusion
calcium
ATP
verapamil
superoxide dismutase
magnesium
