摘要
为探讨细胞角蛋白7（CK7）在大肠癌细胞的表达及其意义，取大肠癌患者标本40例，分别取癌组织及癌旁组织，石蜡包埋后切片，应用CK7单克隆抗体进行免疫组织化学染色，经图像分析系统对阳性细胞进行图像分析。应用统计学方法分析所得结果。结果显示，大肠癌标本CK7可见阳性表达，阳性表达的分布位置以细胞质为主，为棕黄色颗粒状。大肠癌细胞中CK7表达率为100％，而癌旁正常结畅组织均为阴性。结果表明，大肠癌细胞中CK7可作为大肠癌的特异性表面标志物应用于大肠癌的诊断。
The objective of the study was to evaluate the cytokeratin7 （CK7） expression in colorectal cancer cells（CCC） and its significance. Specimen from colorectal cancer and adjacent tissues of colorectal cgncer of 40 patients were collected,imbed with paraffin, then sected, stained with immunohistochemical method by using monoclonal antibody against CK7,and the positive cells were analyzed by image analysis system. And applied statistical methods to analyze the results. As results,the positive expression of the CK7 mainly distributed in the cytoplasm appeared as brown-yellow particles;CK7 expression rate in CCC was 100% ,while that in the adjacent normal tissue was negative. It is suggested that CK7 can be applied as special surface marker in the diagnosis of colorectal cancer.
出处
《中国肛肠病杂志》
2011年第9期9-10,共2页
Chinese Journal of Coloproctology
关键词
大肠癌
细胞角蛋白7
蛋白表达
Colorectal cancer
Cytokeratin 7
Protein expression
