摘要
在单因素试验的基础上,利用响应面分析法对荷叶生物碱盐的提取工艺进行优化。研究加热回流法的提取温度、提取时间、料液比、磷酸体积分数对荷叶生物碱盐提取效果的影响。结果表明,提取最佳工艺条件为提取温度92.7℃、提取时间2.28h、料液比1:24.4(g/mL)、磷酸体积分数0.27%,在此条件下,荷叶生物碱盐的提取率可达5.313mg/g,与理论预测值5.372mg/g的相对误差为1.11%,说明该模型具有较好的分析能力。
Response surface methodology was employed to optimize the extraction of alkaloid salt from lotus leaf based on one-factor-at-a-time experiments.The influences of extraction temperature,extraction time,solid-to-liquid ratio and phosphoric acid concentration on the extraction of alkaloid salt were studied.The results indicated the optimum conditions for alkaloid salt extraction were obtained as follows： temperature 92.7 ℃,extraction time 2.28 h,solid-to-liquid ratio 1：24.4（g/mL）,and 0.27%（V/V） phosphoric acid as the extraction solvent.Under these conditions,and the experimental yield of alkaloid salt was 5.313 mg/g,which was in good agreement with the predicted yield of 5.372 mg/g with a relative error of 1.11%.
出处
《食品科学》
CAS
CSCD
北大核心
2011年第18期54-58,共5页
Food Science
关键词
荷叶生物碱盐
得率
响应面分析
alkaloid salt
extraction yield
response surface analysis
