摘要
目的:从DNA水平检测PTEN基因在肺癌组织中的表达,探讨PTEN基因的表达与肺癌发生发展的关系。方法:应用原位PCR技术检测56例肺癌石蜡标本PTEN基因DNA表达。结果:①PTEN DNA表达阳性信号主要位于癌细胞核,胞浆中亦有少量表达,阳性产物呈棕黄色弥漫分布;②不同性别患者的PTEN基因的表达不同,但差异甚微;③PTEN DNA表达在非小细胞肺癌中的阳性率明显高于小细胞肺癌中的表达阳性率;④分化不良的肺癌PTEN基因表达阳性率低于分化良好和中等分化者;⑤19例有淋巴结转移的肺癌患者PTEN基因表达明显低于37例无淋巴结转移者。结论:PTEN基因在肺癌组织中的表达较正常肺组织中的表达有所降低,说明PTEN基因的低表达可能在肺癌的发生发展过程中起重要作用,且该基因的表达与肺癌患者的性别无显著相关,而与肺癌的病理类型、组织分化程度、临床分期,以及有无淋巴结转移密切相关。
Objective： To detect expression of PTEN gene at lung cancer tissue and probe the relationships between PTEN expression and lung cancer.Methods： The expression of PTEN DNA was detected by in situ PCR.Results： ①The expression of PTEN DNA were mainly situated in nucleus,and partly in cytoplasm.The positive signal were buffy and suffusing distribution.②The expression of PTEN gene were different in gender,but the difference were minute.③The positive expression rate of PTEN DNA in NSCLC was higher than that in SCLC.④The positive expression rate of PTEN in poorly differentiated lung cancer tissue was lower than that in secondary-differentiated and well-differentiated tissue.⑤Expression of PTEN in 19 patients with lymph node metastasis was obviously lower than that in 37 without no-lymph node metastasis.Conclusion： Expression of PTEN gene in lung carcinoma was lower than that in normal lung tissue,which indicated that down-regulation expression of PTEN possibly play an important role in carcinogenesis and development.And expression of PTEN has no correlation with gender of lung cancer patients,while correlated with classify,differentiation,clinical stage and lymph node metastasis of lung cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第9期1270-1273,共4页
Journal of Nanjing Medical University(Natural Sciences)
