摘要
目的:观察麦冬不同提取物对过氧化氢诱导的人脐静脉内皮细胞(HUVEC)间黏附分子-1(ICAM-1)和VEGF、Bcl-2表达的影响。方法:体外培养HUVEC,用过氧化氢(H202)制造HUVEC损伤模型。以四甲基偶氮唑蓝(MTT)比色法检测细胞存活数量,用流式细胞仪检测HUVEC表面ICAM-1的表达量;免疫细胞化学方法检测HUVEC的VEGF、Bcl-2的分布情况。结果:模型组较正常对照组细胞增殖活性明显降低(P<0.01)。与模型组相比,经麦冬水提物、正丁醇提取物处理组细胞增殖活性明显增加(P<0.05,P<0.01)。流式细胞仪检测显示正丁醇提取物可降低过氧化氢增加的ICAM-1基因的表达。Bcl-2的表达,模型组明显低于正常对照组,而正丁醇组表达明显高于模型组(P<0.01)。VEGF的表达,模型组明显高于正常对照组,麦冬水提物、正丁醇提取物处理组高于模型组(P<0.05,P<0.01)。结论:麦冬提取物具有抗凋亡、促增殖、降低细胞间黏附分子-1表达的作用,尤以正丁醇提取物效果更为显著。
Objective： To investigate the influence of different Ophiopogon Japonicus extracts on expression of intercellular adhesion molecule -1 （ICAM-1） and VEGF, Bcl-2 in damaged human umbilical venous endothelial cell （HUVEC） induced by hydrogen peroxide. Methods： HUVECs were cultured in vitro, and HUVEC damage model was induced by using hydrogen peroxide （H202）. Blue methyl thiazolyl tetrazolium （MTT） method was used to detect the number of survival cells, the expression of ICAM-1 on the surface of HUVEC was analyzed by flow cytometry and immunocytochemical method was used to detect the distribution of VEGF, Bcl-2 in HUVEC. Results： Compared with the normal group, the cell proliferate activity of model group was significantly decreased （P 〈0.01）, while after being treated by water extract and butanol extract from Ophiopogon Japonicus, it was significantly increased compared with the model group （P 〈0.05, P 〈0.01）. The measurement of flow cytometry showed that the n-butanol extract could reduce the increased gene expression of ICAM-1 caused by hydrogen peroxide. For Bcl-2 expression, the model group was much less than the normal control group, and Ophiopogon Japonicus extract group was significantly higher than the model group （P 〈0.01）. For VEGF expression, the model group was much higher than the normal group, and aqueous extract and n-butanol extract of Ophiopogon Japonicus group was higher than the model group （P〈0.05, P 〈0.01）. Conclusion： Ophiopogon Japonicus extract, especially the extract by n-Butanol could be used for anti-apoptotic, promoting proliferation, reducing expression of ICAM-1.
出处
《现代生物医学进展》
CAS
2011年第18期3421-3426,共6页
Progress in Modern Biomedicine
基金
Nature Science Foundation of China(NSFC),contract/grant number:30300464~~
关键词
麦冬
血管内皮细胞
VEGF
过氧化氢
ICAM-1
Bcl-2
Ophiopogon Japonicus
Human umbilical venous endothelial cell （HUVEC）
VEGF
Hydrogen peroxide （H2O2）
ICAM- 1
Bcl-2
