摘要
选取健康未孕的成年沂蒙黑山羊,按发情周期(间情期、发情前期、发情期、发情后期)宰杀取卵巢,采用实时荧光定量差异显示PCR(real-time qRT-PCR)技术,以GAPDH为持家基因,对处于发情周期不同阶段的沂蒙黑山羊卵巢组织内的卵泡刺激素受体(FSHR)、黄体生成素受体(LHR)基因进行了mRNA水平上的相对定量分析。发现FSHR mRNA、LHR mRNA在黑山羊发情周期的卵巢中都有表达。FSHRmRNA的相对表达量依次为0.618 7±0.294 42、1.290 2±0.290 24、2.575 9±0.458 80、0.790 8±0.261 93,其中发情期最高,间情期最低,两者差异极显著(P<0.01)。LHR mRNA的相对表达量依次为2.284 0±0.146 22、0.508 9±0.0789 9、0.340 3±0.045 38、2.859 2±0.205 66,其中发情后期相对表达量最高,发情期最低,两者差异极显著(P<0.01)。结果表明,FSH、LH在黑山羊的整个发情周期中是相互作用、相互影响的。
In order to examine the mRNA levels of FSHR gene and LHR gene in ovaries of four estrous cycle phases in Yimeng Black goats,healthy,non-pregnant adult goats were killed in this study according to the estrous cycle for normal ovaries and real time fluorescence quantitative PCR method（real-time qRT-PCR） with GAPDH as reference gene was established.The results showed that FSHR gene and LHR gene were expressed in the ovaries of Yimeng Black goats during the four estrous cycle phases.The relative expression of FSHR was respectively 0.618 7±0.294 42,1.290 2±0.290 24,2.575 9±0.458 80 and 0.790 8±0.261 93.The FSHR mRNA relative expression was the highest in estrus and the lowest in diestrus with a very significant difference between two phases（P0.01）.The relative expression of LHR was respectively 2.284 0±0.146 22,0.508 9±0.078 99,0.340 3±0.045 38 and 2.859 2±0.205 66.The LHR mRNA relative expression was the highest in metestrus and the lowest in estrus with a very significant difference between two phases（P0.01）.These results indicated that there is a close interaction between FSH and LH during the whole estrous cycle of Yimeng Black goats.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第9期954-959,共6页
Chinese Veterinary Science
基金
山东省科技攻关项目(2006GG2209004)
国家自然科学基金项目(30871832)
