摘要
目的通过对人类白细胞抗原(HLA)-DR基因的检测,探讨哮喘儿童HLA-DRB基因型基因多态性与儿童哮喘易感性的关系。方法将无血缘关系的84名哮喘儿童(哮喘组)和168名无哮喘和特应性疾病的健康个体(对照组)纳入研究。采用PharmaciaUniCAP系统检测哮喘儿童的血清总IgE水平,同时完成10种吸入性过敏原皮肤实验。应用基因芯片法检测HLA-DR的21个基因位点。结果支气管哮喘组HLA-DRB1*070X基因和HLA-DRB1*11XX基因频率(分别为2.98%和13.69%)高于对照组(分别为0.3%和5.95%,x^2=6.915,P<0.05和x^2=9.478,P<0.01),优势比(OR)分别为10.57(95%CI 1.215～91.986)和2.79(95%CI 1.429～5.449);HLA-DRB3(52)*010X基因频率在哮喘组为7.14%,低于对照组的13.99%(x^2=5.854,P<0.05),OR为0.429(95%CI 0.214～0.862)。结论 HLA-DRB1*070X基因和HLA-DRB1*11XX基因与哮喘儿童易感性相关;HLA-DRB3(52)*010X基因则为抗性基因。
Objective To investigate the association between human leukocyte antigen DRB （HLA-DRB） gene polymorphisms and asthma in children. Methods Eighty-four unrelated asthmatic individuals and one hundred and sixty-elght healthy controls without asthma and atopy were involved in the study. All asthmatics had their serum total IgE levels measured with UniCAP Pharmaeia system, and skin-prick test with ten kinds of inhalant allergens were taken among the asthmafies. HLA oligonueleotide array was used to twenty-one gene frequeneics of HLA-DR. Results The frequeneics of HLA-DRB1 ＊ 070X allele and HLA-DRB1 ＊ llXX allele among the asthmatics were 2. 98% and 13.69%, significantly higher than those in healthy controls （0. 3% and 5.95%, X^2 = 6. 915, P 〈 0. 05 and X^2 = 9. 478, P 〈 0. 01 ） ）, Odds ratios （Ors） were 10. 57 （95 % CI： I. 215 - 91. 986 ） and 2. 79 （ 95 % C1：1. 429 ~ 5. 449 ） respectively. HLA-DRB3 （52） ＊ 010Xallele were significantly decreased in asthmatics compared to healthy controls （ 13.99%, X^2 = 5. 854, P 〈 0. 05）, Ors were 0. 429 （95 % CI：0. 214 -0. 862）. Conclusion HLA-DRB1 ＊ 070X al- lele and HLA-DRB1 ＊ 11XX allele were implicated in susceptibility to asthma, HLA-DRB3 （52） ＊ 010X allele might confer protection against asthma.
出处
《中国临床新医学》
2011年第9期829-831,共3页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
广西卫生厅自筹经费科研课题(编号:桂卫科Z2009167)
