摘要
实验采用不对称竞争性PCR技术,测定16只不同毛色藏羊Agouti基因的拷贝重复数,对记录所得Agouti基因连接点的比值进行分析。对样品（n=16）的Agouti基因拷贝数进行分析,区分未知样品Agouti基因的拷贝数。10只全白藏羊的拷贝数在3-6个之间,其余不同毛色6只藏羊均为2个拷贝数。实验证明：不对称竞争性PCR技术检测Agouti基因拷贝重复数变异的方法,可以直接对产物进行定量,具有良好的稳定性和特异性,而且操作简单、安全,自动化程度高,不易产生污染,不需昂贵设备,适合在普通实验室检测。
The Agouti gene plays an important role in pigment synthesis in domestic animals.In this study,a powerful method for quantification of duplicated copies based on ABI3130xl sequencing and asymmetric competitive PCR was described.An asymmetric competitive PCR copy number assay was established for the copy number variation（CNV） detection by the ratio of the area under the 242-bp（junction point） peak compared with the total area represented by both peaks（junction point and breakpoint）.The results showed that the CNV in Agouti gene of 16 Tibetan sheep were estimated.The CNV in Aagouti gene of six different color Tibetan sheep were 2 copies;while for ten white Tibetan sheep were 3,4 and 5 copies,respectively.The asymmetric competitive PCR copy number assay is a simple,rapid method with high resolution and stability,and it can be used in common laboratories.
出处
《中国草食动物》
2011年第5期5-8,共4页
China Herbivores
基金
国家绒毛用羊产业技术体系分子育种岗位(nycytx-40-2)
