摘要
将猪去氧胆酸粗品形成甲酯,然后用苯结晶,分离出猪去氧胆酸甲酯,再利用NaOH的乙醇-水溶液进行水解,得到纯猪去氧胆酸;对猪去氧胆酸粗品中含有的主要杂质进行了结晶分离,通过IR,1H NMR,13C NMR,DEPT谱,HDMS,DSC/TGA和2D-NMR进行了结构鉴定,确定其主要杂质为猪胆酸。
Methyl hyodeoxycholate was obtained from impure hyodeoxycholic acid,and it was crystallized in benzene.The pure methyl hyodeoxycholate was then hydrolyzed by sodium hydroxide in aqueous ethanol,and pure hyodeoxycholic acid was obtained.The major impurity was separated by column chromatography,and it was confirmed as hyocholic acid by means of IR,1H NMR,13C NMR,DEPT,HDMS,DSC/TGA,and 2D nuclear resonances.
出处
《化学世界》
CAS
CSCD
北大核心
2011年第9期543-546,共4页
Chemical World
关键词
猪去氧胆酸
纯化
杂质
结构鉴定
hyodeoxycholicy acid
purification
impurity
structure confirmation
